Human antibodies from immunized donors are protective against anthrax toxin in vivo.

Nature biotechnology ·Vol. 21 ·No. 11 ·2003-11-00 ·Pages 1305-6

Wild MA, Xin H, Maruyama T, Nolan MJ, Calveley PM, Malone JD, Wallace MR, Bowdish KS

Abstract

A panel of Fabs that neutralize anthrax toxin in vitro was selected from libraries generated from human donors vaccinated against anthrax. At least two of these antibodies protect rats from anthrax intoxication in vivo. Fabs 83K7C and 63L1D bind with subnanomolar affinity to protective antigen (PA) 63, and Fab 63L1D neutralizes toxin substoichiometrically, inhibits lethal factor (LF) interaction with PA63 and binds to a conformational epitope formed by PA63.

MeSH Terms

Animals Antibodies/blood,immunology Antigens, Bacterial Bacterial Toxins/antagonists & inhibitors,immunology,toxicity Blood Donors Humans Immunity/physiology Immunization/methods Immunoglobulin Fab Fragments/blood,immunology Peptide Library Rats

Chemicals

Antibodies Antigens, Bacterial Bacterial Toxins Immunoglobulin Fab Fragments Peptide Library anthrax toxin

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Wild Martha A

Alexion Antibody Technologies, Inc., 3985 Sorrento Valley Blvd., San Diego, California 92121, USA.

Xin Hong

Maruyama Toshiaki

Nolan Mary Jean

Calveley Peter M

Malone John D

Wallace Mark R

Bowdish Katherine S

Article Info

Journal

Nature biotechnology

Abbr.

Nat Biotechnol

ISSN

1087-0156

DOI

10.1038/nbt891

Published

2003-11-00

Epub

2003-00-12

Pages

1305-6

Language

English

Region

United States

NLM ID

9604648

Subset

External Links

PubMed source DOI: 10.1038/nbt891 View journal details

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Human antibodies from immunized donors are protective against anthrax toxin in vivo.

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