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PMID: 14532344 Published · ppublish English Journal Article

Evaluation of a multilocus sequence typing system for Staphylococcus epidermidis.

Journal of medical microbiology ·Vol. 52 ·No. Pt 11 ·2003-11-00 ·Pages 989-998

Wang XM, Noble L, Kreiswirth BN, Eisner W, McClements W, Jansen KU, Anderson AS

Abstract

Staphylococcus epidermidis is a significant cause of nosocomial disease. However, the taxonomy of this pathogen, particularly at subspecies level, is unclear. A multilocus sequence typing (MLST) scheme has therefore been investigated as a tool to elucidate taxonomic relationships within this group, based on genetic relatedness. DNA sequences for internal fragments of seven housekeeping genes were compared in 47 geographically and temporally diverse S. epidermidis isolates that were obtained from clinical infections. Twenty-three different allelic profiles were detected; 17 of these were represented by single strains and the largest profile group contained 17 isolates. Diversity of the same collection of isolates was investigated by using PFGE of SmaI-digested genomic DNA to test the discrimination and validity of the MLST approach. Isolates within the largest profile group were resolved into four distinct PFGE clusters on the basis of their SmaI digest patterns. Isolates within other profile groups that contained multiple isolates had matching PFGE SmaI patterns within each group. It appears that MLST is an effective method for grouping S. epidermidis strains at the subspecies level; however, it is not as discriminatory as it has been for other species for which MLST schemes have been established and, used alone, would not be a useful method for epidemiological studies. In addition, it was demonstrated that this method was effective for confirming the identity of S. epidermidis CoNS (coagulase-negative) isolates.

MeSH Terms
Bacterial Typing Techniques/methods Base Sequence Electrophoresis, Gel, Pulsed-Field Methicillin Resistance Molecular Sequence Data Staphylococcus epidermidis/classification,drug effects,genetics
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Wang Xin-Min
Merck and Co. Inc., PO Box 4 WP 26-265, Sumneytown Pike, West Point, PA 19486, USA 2Merck and Co. Inc., PO Box 2000 RY80Y 230, 126 E. Lincoln Ave., Rahway, NJ 07065, USA 3PHRI TB Center, Public Health Research Institute, 225 Warren Street, Newark, NJ 07103, USA.
Noble Liliane
Merck and Co. Inc., PO Box 4 WP 26-265, Sumneytown Pike, West Point, PA 19486, USA 2Merck and Co. Inc., PO Box 2000 RY80Y 230, 126 E. Lincoln Ave., Rahway, NJ 07065, USA 3PHRI TB Center, Public Health Research Institute, 225 Warren Street, Newark, NJ 07103, USA.
Kreiswirth Barry N
Merck and Co. Inc., PO Box 4 WP 26-265, Sumneytown Pike, West Point, PA 19486, USA 2Merck and Co. Inc., PO Box 2000 RY80Y 230, 126 E. Lincoln Ave., Rahway, NJ 07065, USA 3PHRI TB Center, Public Health Research Institute, 225 Warren Street, Newark, NJ 07103, USA.
Eisner William
Merck and Co. Inc., PO Box 4 WP 26-265, Sumneytown Pike, West Point, PA 19486, USA 2Merck and Co. Inc., PO Box 2000 RY80Y 230, 126 E. Lincoln Ave., Rahway, NJ 07065, USA 3PHRI TB Center, Public Health Research Institute, 225 Warren Street, Newark, NJ 07103, USA.
McClements William
Merck and Co. Inc., PO Box 4 WP 26-265, Sumneytown Pike, West Point, PA 19486, USA 2Merck and Co. Inc., PO Box 2000 RY80Y 230, 126 E. Lincoln Ave., Rahway, NJ 07065, USA 3PHRI TB Center, Public Health Research Institute, 225 Warren Street, Newark, NJ 07103, USA.
Jansen Kathrin U
Merck and Co. Inc., PO Box 4 WP 26-265, Sumneytown Pike, West Point, PA 19486, USA 2Merck and Co. Inc., PO Box 2000 RY80Y 230, 126 E. Lincoln Ave., Rahway, NJ 07065, USA 3PHRI TB Center, Public Health Research Institute, 225 Warren Street, Newark, NJ 07103, USA.
Anderson Annaliesa S
Merck and Co. Inc., PO Box 4 WP 26-265, Sumneytown Pike, West Point, PA 19486, USA 2Merck and Co. Inc., PO Box 2000 RY80Y 230, 126 E. Lincoln Ave., Rahway, NJ 07065, USA 3PHRI TB Center, Public Health Research Institute, 225 Warren Street, Newark, NJ 07103, USA.
Article Info
Journal
Journal of medical microbiology
Abbr.
J Med Microbiol
ISSN
0022-2615
DOI
10.1099/jmm.0.05360-0
Published
2003-11-00
Pages
989-998
Language
English
Region
England
NLM ID
0224131
Subset
IM
Databases
GENBANK
AY163262, AY163263, AY163264, AY163265, AY163266, AY163267, AY163268, AY163269, AY163270, AY163271, AY163272, AY163273, AY163274, AY163275, AY163276, AY163277, AY163278, AY163279, AY163280, AY163281, AY163282, AY163283, AY163284, AY163285, AY163286, AY163287, AY163288, AY163289, AY163290, AY163291, AY163292, AY163293, AY163294, AY163295, AY163296, AY163297, AY163298, AY163299, AY163300, AY163301, AY163302, AY163303, AY163304, AY163305, AY163306, AY163307, AY163308, AY163309, AY163310, AY163311, AY163312, AY323453, AY323454, AY323455, AY323456, AY323457, AY323458, AY323459
External Links
PubMed source DOI: 10.1099/jmm.0.05360-0 View journal details
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