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PMID: 14517243 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Phosducin-like proteins in Dictyostelium discoideum: implications for the phosducin family of proteins.

The EMBO journal ·Vol. 22 ·No. 19 ·2003-10-01 ·Pages 5047-57

Blaauw M, Knol JC, Kortholt A, Roelofs J, Ruchira, Postma M, Visser AJ, van Haastert PJ

Abstract

Retinal phosducin is known to sequester transducin Gbetagamma, thereby modulating transducin activity. Phosducin is a member of a family of phosducin-like proteins (PhLP) found in eukaryotes. Phylogeny of 33 phosducin-like proteins from metazoa, plants and lower eukaryotes identified three distinct groups named phosducin-I-III. We discovered three phlp genes in Dictyostelium, each encoding a phosducin-like protein of a different group. Disruption of the phlp1 gene strongly impaired G-protein signalling, apparently due to mislocalization of Gbetagamma in phlp1-null cells. GFP-Gbeta and GFP-Ggamma are membrane associated in wild-type cells, but cytosolic in phlp1-null cells. Phlp2 disruption is lethal due to a synchronous collapse of the cells after 16-17 cell divisions. Phlp3 disruptants show no abnormal phenotype. These results establish a role for phosducin-like proteins in facilitating folding, localization or function of proteins, in addition to modulating G-protein signalling.

MeSH Terms
Amino Acid Sequence Animals Carrier Proteins/genetics,metabolism Dictyostelium/metabolism Genes, Reporter Molecular Sequence Data Multigene Family/physiology Nerve Tissue Proteins/genetics,metabolism Phylogeny Sequence Analysis, Protein
Chemicals
Carrier Proteins Nerve Tissue Proteins
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Blaauw Mieke
Department of Biochemistry, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands.
Knol Jaco C
Kortholt Arjan
Roelofs Jeroen
Ruchira
Postma Marten
Visser Antonie J W G
van Haastert Peter J M
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/cdg508
Published
2003-10-01
Pages
5047-57
Language
English
Region
England
NLM ID
8208664
PMCID
PMC204491
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/cdg508 PMC Full Text View journal details
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