Location of enzymes in Azotobacteragilis.

Journal of bacteriology ·Vol. 83 ·1962-01-00 ·Pages 158-68

ROBRISH SA, MARR AG

Abstract

Robrish, Stanley A. (University of California, Davis) and Allen G. Marr. Location of enzymes in Azotobacter agilis. J. Bacteriol. 83: 158-168. 1962.-If the cells of Azotobacter agilis are disrupted by osmotic shock, respiratory enzymes and the compounds characteristic of cell wall and cytoplasmic membrane are recovered almost completely in large particles. The large particles obtained by osmotic shock were found by electron microscopy to consist of cell wall, cell membrane, and an internal membrane appearing as either vesicles or tubules in section. These envelopes are free of all the soluble cytoplasmic material and are essentially free of ribosomes. Small particles obtained by osmotic shock are ribosomes; small particles obtained by sonic oscillation consist of both ribosomes and amorphous material, presumably fragments of the envelope.

Keywords

AZOTOBACTER/metabolism ENZYMES/metabolism

MeSH Terms

Azotobacter/metabolism Cell Membrane Cytoplasm Enzymes/metabolism Microscopy, Electron

Chemicals

Enzymes

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

ROBRISH S A

MARR A G

References (25)

25 references, click to expand

Elimination of substances interfering with nucleic acids estimation.
Biochim Biophys Acta. 1959 Jan;31(1):195-207 PMID: 13628621
Method for the determination of hexosamines in tissues.
J Biol Chem. 1953 Oct;204(2):553-63 PMID: 13117829
The structure of Rhodospirillum rubrum.
J Biophys Biochem Cytol. 1959 Oct;6:277-84 PMID: 14401694
A membranous component of the cytoplasm in Streptomyces coelicolor.
J Biophys Biochem Cytol. 1959 Dec;6:515-6 PMID: 13828387
Sonic disruption of Azotobacter vinelandil.
J Bacteriol. 1957 Jul;74(1):79-86 PMID: 13462964
Electrophoretic analysis of the macromolecular nucleoprotein particles of mammalian cytoplasm.
Cancer Res. 1954 Jun;14(5):360-6 PMID: 13160964
Disposition of membranes in Alcaligenes faecalis.
J Bacteriol. 1960 Nov;80:659-64 PMID: 13688633
The chemical composition of the protoplast membrane of Micrococcus lysodeikticus.
Biochim Biophys Acta. 1958 Jul;29(1):21-9 PMID: 13560442
Studies of the bacterial cell wall. II. Methods of preparation and some properties of cell walls.
Biochim Biophys Acta. 1951 Jul;7(2):177-97 PMID: 14858403
Fine structure of the nucleoid and internal membrane systems of Streptomyces.
J Bacteriol. 1959 Aug;78:272-81 PMID: 13835355
Sub-cellular particles and the nicotinic acid hydroxylase system in extracts of.
Biochim Biophys Acta. 1959 Aug;34:354-72 PMID: 13848461
Enzyme localization in bacteria.
Annu Rev Microbiol. 1960;14:241-60 PMID: 13767101
SYNTHESIS OF NASCENT PROTEIN BY RIBOSOMES IN ESCHERICHIA COLI.
Proc Natl Acad Sci U S A. 1959 Sep;45(9):1437-47 PMID: 16590524
Submicroscopic particles in extracts of Azotobacter agilis.
J Bacteriol. 1958 Mar;75(3):243-52 PMID: 13513592
Localization of enzymes in the microbial cell.
Bacteriol Rev. 1956 Jun;20(2):67-93 PMID: 13341821
Studies on the macro-molecular organization of microbial cells.
Arch Biochem Biophys. 1952 Jul;38:245-60 PMID: 12997099
A chlorophyll-containing cell fraction from the blue-green alga, Anabaena variabilis.
J Biophys Biochem Cytol. 1960 Jun;7:583-4 PMID: 14445620
Hydrogenase and nitrogenase in Azotobacter.
J Bacteriol. 1953 May;65(5):511-7 PMID: 13069414
Association of carotenoid pigments with protein components in non-photosynthetic bacteria.
Biochim Biophys Acta. 1955 Apr;16(4):482-8 PMID: 14389264
Characterization of the protoplasmic constituents of bacillus megaterium.
J Bacteriol. 1953 Dec;66(6):696-702 PMID: 13117796
The effect of x rays on the macromolecular organization of Escherichia coli.
J Bacteriol. 1954 Feb;67(2):191-97 PMID: 13129212
The estimation of phosphorus.
Biochem J. 1940 Jun;34(6):858-65 PMID: 16747230
Electron microscopy of ultrathin sections of bacteria. III. Cell wall, cytoplasmic membrane, and nuclear material.
J Bacteriol. 1959 Jul;78(1):96-104 PMID: 13672914
The fine structure of the nuclear material of a blue-green alga, Anabaena cylindrica Lemm.
J Biophys Biochem Cytol. 1960 Dec;8:813-23 PMID: 13715795
The fine structure of vegetative cells of Bacillus subtilis.
Exp Cell Res. 1961 Jan;22:73-85 PMID: 13705983

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.83.1.158-168.1962

Published

1962-01-00

Pages

158-68

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC314802

Subset

External Links

PubMed source DOI: 10.1128/jb.83.1.158-168.1962 PMC Full Text View journal details

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