Home LiteratureArticle Details
PMID: 144916 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Oligomycin-dependent ionophoric protein subunit of mitochondrial adenosinetriphosphatase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 10 ·1977-10-00 ·Pages 4306-10

Criddle RS, Packer L, Shieh P

Abstract

A proteolipid isolated from yeast mitochondrial adenosinetriphosphatase (subunit 9) (ATP phosphohydrolase; EC 3.6.1.3) by chloroform/methanol extraction has been shown to discharge photo-induced potentials across a planar phospholipid membrane containing bacteriorhodopsin. Oligomycin, a specific inhibitor of oxidative phosphorylation which binds to this protein, allows the potential gradient to be reestablished. When proteolipid was isolated from an oligomycin-resistant strain, ionophoric activity was still obtained but the effect was not reversed by oligomycin. These studies suggest that the hydrophobic subunit-9 polypeptide is the ionophoric component linking ATP synthesis (hydrolysis) with proton translocation.

MeSH Terms
Adenosine Triphosphatases Bacteriorhodopsins Chemical Phenomena Chemistry Drug Resistance, Microbial Filtration Fungal Proteins/analysis Ionophores/analysis Lipids Membranes, Artificial Mitochondria/enzymology Oligomycins/pharmacology Saccharomyces cerevisiae/enzymology Time Factors
Chemicals
Fungal Proteins Ionophores Lipids Membranes, Artificial Oligomycins Bacteriorhodopsins Adenosine Triphosphatases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Criddle R S
Packer L
Shieh P
References (25)
25 references, click to expand
  1. A coupling factor from sarcoplasmic reticulum required for the translocation of Ca2+ ions in a reconstituted Ca2+ATPase pump.
    J Biol Chem. 1975 Sep 25;250(18):7533-4 PMID: 126239
  2. Identification of the dicyclohexylcarbodiimide-reactive protein component of the adenosine 5'-triphosphate energy-transducing system of Escherichia coli.
    J Bacteriol. 1975 Nov;124(2):870-83 PMID: 126994
  3. Separate effects of mercurial compounds on the ionophoric and hydrolytic functions of the (Ca++ +Mg++)-ATPase of sarcoplasmic reticulum.
    J Membr Biol. 1975 Dec 4;25(1-2):65-74 PMID: 129568
  4. Localization of ionophore activity in a 20,000-dalton fragment of the adenosine triphosphatase of Sarcoplasmic reticulum.
    J Biol Chem. 1976 Jul 10;251(13):4147-54 PMID: 132445
  5. Assembly of the mitochondrial membrane system XVI. Modified form of the ATPase proteolipid in oligomycin-resistant mutants of Saccharomyces cerevisiae.
    FEBS Lett. 1976 Jun 15;65(3):391-5 PMID: 133820
  6. Antibiotics as tools for metabolic studies. I. A survey of toxic antibiotics in respiratory, phosphorylative and glycolytic systems.
    Arch Biochem Biophys. 1958 Dec;78(2):587-97 PMID: 13618041
  7. Affinity labeling of yeast mitochondrial adenosine triphosphatase by reduction with [3H]borohydride.
    Arch Biochem Biophys. 1977 Aug;182(2):587-600 PMID: 143240
  8. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  9. Proton translocation by ATPase and bacteriorhodopsin.
    Fed Proc. 1977 May;36(6):1815-8 PMID: 15875
  10. Isolation of a chloroplast N,N'-dicyclohexylcarbodiimide-binding proteolipid, active in proton translocation.
    Proc Natl Acad Sci U S A. 1977 Jun;74(6):2375-8 PMID: 18736
  11. The electromechanochemical model for energy coupling in mitochondria.
    Biochim Biophys Acta. 1974 Apr 30;346(1):27-78 PMID: 4151654
  12. Promitochondria of anaerobically grown yeast. I. Isolation and biochemical properties.
    Biochemistry. 1969 Jan;8(1):322-34 PMID: 4238010
  13. Assembly of the mitochondrial membrane system. V. Properties of a dispersed preparation of the rutamycin-sensitive adenosine triphosphatase of yeast mitochondria.
    J Biol Chem. 1971 Dec 10;246(23):7328-36 PMID: 4256832
  14. Alterations in mitochondrial adenosine triphosphatase activity resulting from mutation of mitochondrial deoxyribonucleic acid.
    J Biol Chem. 1973 May 10;248(9):3004-11 PMID: 4267040
  15. Hypothesis: cation-translocating adenosine triphosphatase models: how direct is the participation of adenosine triphosphate and its hydrolysis products in cation translocation?
    FEBS Lett. 1973 Jul 15;33(3):267-74 PMID: 4269644
  16. Assembly of the mitochondrial system. Purification of a mitochondrial product of the ATPase.
    Proc Natl Acad Sci U S A. 1973 Nov;70(11):3155-9 PMID: 4274131
  17. A Ca++-dependent and -selective ionophore as part of the Ca++ plus Mg++-dependent adenosinetriphosphatase of sarcoplasmic reticulum.
    Proc Natl Acad Sci U S A. 1974 Sep;71(9):3522-6 PMID: 4279408
  18. Assembly of the mitochondrial membrane system. I. Characterization of some enzymes of the inner membrane of yeast mitochondria.
    J Biol Chem. 1969 Sep 25;244(18):5020-6 PMID: 4309989
  19. Direct measurement of electric current generation by cytochrome oxidase, H+-ATPase and bacteriorhodopsin.
    Nature. 1974 May 24;249(455):321-4 PMID: 4366965
  20. Proteolipids from membrane systems.
    Ann N Y Acad Sci. 1972 Jun 20;195:86-107 PMID: 4504108
  21. Assembly of the mitochondrial membrane system. 8. Properties of the products of mitochondrial protein synthesis in yeast.
    J Biol Chem. 1972 Oct 25;247(20):6517-23 PMID: 4561938
  22. Solubility of the intramitochondrially synthesized protein and other membrane proteins of rat liver mitochondria in acidic or neutral chloroform-methanol.
    Biochem Biophys Res Commun. 1973 Sep 5;54(1):9-16 PMID: 4741581
  23. Protein-induced conductivity changes in black lipid membranes and protein aggregation.
    FEBS Lett. 1977 Feb 15;74(1):47-9 PMID: 556999
  24. Purification of the carbodiimide-reactive protein component of the ATP energy-transducing system of Escherichia coli.
    J Biol Chem. 1976 Nov 10;251(21):6630-7 PMID: 789371
  25. Photo-induced potentials across a polymer stabilized planar membrane, in the presence of bacteriorhodopsin.
    Biochem Biophys Res Commun. 1976 Jul 26;71(2):603-9 PMID: 962942
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.10.4306
Published
1977-10-00
Pages
4306-10
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC431929
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.74.10.4306 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2