Electron microscopy of lysosome-rich fractions from rat thymus isolated by density-gradient centrifugation before and after whole-body x-irradiation.

The Journal of cell biology ·Vol. 13 ·1962-05-00 ·Pages 253-60

RAHMAN YE

Abstract

Fractions from rat thymuses were isolated by sucrose density-gradient centrifugation, before and after 1000 r whole-body x-irradiation, and examined by electron microscopy. Cytochrome oxidase and acid phosphatase activities of these fractions were tested as well. Electron-opaque bodies with diameters ranging from 0.10 to 0.35 micro, with a mean of 0.25 micro, were found in fractions having high acid phosphatase activity, while the fractions rich in cytochrome oxidase consisted mostly of mitochondria. After irradiation, there was an increased ratio of dense bodies to mitochondria. These particles are considered to be lysosomes similar to those identified in other rat tissues. Their relationship to the mitochondria is discussed.

Keywords

CENTRIFUGATION MICROSCOPY ELECTRON RADIATION EFFECTS/experimental THYMUS GLAND/anatomy and histology

MeSH Terms

Animals Centrifugation Centrifugation, Density Gradient Electron Transport Complex IV Electrons Lysosomes Microscopy Microscopy, Electron Mitochondria Radiation Effects Rats Thymus Gland/anatomy & histology

Chemicals

Electron Transport Complex IV

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

RAHMAN Y E

References (13)

13 references, click to expand

A new approach to the cytochemical localization of enzymes.
Proc R Soc Lond B Biol Sci. 1954 Mar 25;142(907):160-9 PMID: 13167062
Pancreatic microsomes; an integrated morphological and biochemical study.
J Biophys Biochem Cytol. 1956 Nov 25;2(6):671-90 PMID: 13398437
The isolation and properties of the specific cytoplasmic granules of rabbit polymorphonuclear leucocytes.
J Exp Med. 1960 Dec 1;112:983-1004 PMID: 13694490
Localization of acid phosphatase activity in hepatic lysosomes by means of electron microscopy.
J Biophys Biochem Cytol. 1961 Apr;9:773-84 PMID: 13697427
The effect of total-body x-irradiation on the thymus and the number of its cells.
Radiat Res. 1961 Jul;15:30-8 PMID: 13715086
Electron-dense bodies in liver tissue of the mouse in relation to the activity of acid phosphatase.
J Ultrastruct Res. 1960 Oct;4:43-57 PMID: 13766001
Segregation of ferritin in glomerular protein absorption droplets.
J Biophys Biochem Cytol. 1960 Apr;7:297-304 PMID: 13821609
The localization of acid phosphatase in rat liver cells as revealed by combined cytochemical staining and electron microscopy.
J Biophys Biochem Cytol. 1961 Oct;11:47-66 PMID: 13908584
Tissue fractionation studies. 4. Comparative study of the binding of acid phosphatase, beta-glucuronidase and cathepsin by rat-liver particles.
Biochem J. 1955 Mar;59(3):433-8 PMID: 14363113
The reaction of the mouse thymus to x-rays measured by changes in organ weight.
Radiat Res. 1955 May;2(3):280-93 PMID: 14372047
The bile canaliculi; study with an electronic microscope.
C R Seances Soc Biol Fil. 1954 Dec;148(23-24):2008-11 PMID: 14379565
Increased activity of acid phosphatase and beta-glucuronidase in the liver and spleen of mice after intraperitoneal administration of various macromolecular substances.
Biochem Pharmacol. 1961 Dec;8:389-97 PMID: 14472187
A microspectrophotometric method for the determination of cytochrome oxidase.
J Biol Chem. 1951 Apr;189(2):665-70 PMID: 14832284

Article Info

Journal

The Journal of cell biology

Abbr.

J Cell Biol

ISSN

0021-9525

DOI

10.1083/jcb.13.2.253

Published

1962-05-00

Pages

253-60

Language

English

Region

United States

NLM ID

0375356

PMCID

PMC2106822

Subset

External Links

PubMed source DOI: 10.1083/jcb.13.2.253 PMC Full Text View journal details

Analysis Services

Electron microscopy of lysosome-rich fractions from rat thymus isolated by density-gradient centrifugation before and after whole-body x-irradiation.

Contact

WeChat Official Account