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PMID: 1445285 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Involvement of extracellular-matrix-degrading metalloproteinases in rabbit aortic smooth-muscle cell proliferation.

The Biochemical journal ·Vol. 288 ( Pt 1) ·1992-11-15 ·Pages 93-9

Southgate KM, Davies M, Booth RF, Newby AC

Abstract

We investigated the influence of two structurally unrelated inhibitors of matrix-degrading metalloproteinases, Ro 31-4724 and Ro 31-7467, on the primary proliferation of smooth-muscle cells from rabbit aortic explants. Both agents inhibited proliferation in a concentration-dependent manner, but did not affect cell viability. Smooth-muscle cells grown out from explants secreted 95 kDa and 72 kDa gelatinase enzymes that were also inhibited in a concentration-dependent manner by Ro 31-4724 and Ro 31-7467. Interstitial collagenase and stromelysin were not detected. We conclude that metalloproteinases are likely to be involved in the initiation of smooth-muscle proliferation.

MeSH Terms
Animals Aorta/cytology Blotting, Western Cell Division/drug effects,physiology Collagenases/analysis Culture Techniques Extracellular Matrix/metabolism Gelatin/metabolism Hydroxamic Acids/pharmacology Immunosorbent Techniques Matrix Metalloproteinase 1 Matrix Metalloproteinase 3 Metalloendopeptidases/analysis,antagonists & inhibitors,metabolism Muscle, Smooth, Vascular/cytology Organophosphorus Compounds/pharmacology Rabbits
Chemicals
Hydroxamic Acids Organophosphorus Compounds Ro 314724 Ro 317467 Gelatin Collagenases Metalloendopeptidases Matrix Metalloproteinase 3 Matrix Metalloproteinase 1
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Southgate K M
Department of Cardiology, University of Wales College of Medicine, Heath Park, Cardiff, U.K.
Davies M
Booth R F
Newby A C
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2880093
Published
1992-11-15
Pages
93-9
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1132084
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2880093 PMC Full Text View journal details
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