Studies on the antigenic properties of complement. I. Demonstration of agglutinating antibodies against guinea pig complement fixed on sensitized sheep erythrocytes.

The Journal of experimental medicine ·Vol. 111 ·1960-01-01 ·Pages 93-106

KLEIN PG, BURKHOLDER PM

Abstract

Evidence is presented to show that guinea pig complement fixed on sensitized sheep red cells acts as a specific agglutinogen. Agglutinating antibodies that react with cell-fixed complement can be produced by immunizing rabbits with a complex of stromata-amboceptor-complement or with guinea pig serum globulin. These agglutinins can be removed by precipitation with guinea pig serum. They are, therefore, distinct from immunoconglutinins.

Keywords

ANTIBODIES COMPLEMENT

MeSH Terms

Animals Antibodies Antigens Complement System Proteins Erythrocytes Guinea Pigs Rabbits Sheep

Chemicals

Antibodies Antigens Complement System Proteins

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

KLEIN P G

BURKHOLDER P M

References (8)

8 references, click to expand

Preserving fluorescein isocyanate for simplified preparation of fluorescent antibody.
Science. 1957 Oct 25;126(3278):839-40 PMID: 13467285
Kinetic studies on immune hemolysis. VII. Decay of EAC'1, 4, 2, fixation of C'3, and other factors influencing the hemolytic action of complement.
J Immunol. 1954 Dec;73(6):443-54 PMID: 13212068
Studies on immune hemolysis: preparation of a stable and highly reactive complex of sensitized erythrocytes and the first component of complement (EAC'1): inactivation of cell-fixed C'1 by some complement reagents.
J Exp Med. 1960 Jan 1;111:77-91 PMID: 13848490
Studies on the antigenic properties of complement. II. Analysis of specific agglutinins against certain components of guinea pig complement fixed on sensitized sheep erythrocytes.
J Exp Med. 1960 Jan 1;111:107-17 PMID: 14409703
Immunological estimation of a component of complement.
Nature. 1958 Jun 14;181(4624):1667-8 PMID: 13566107
The demonstration of an antibody against complement.
J Immunol. 1956 Feb;76(2):112-8 PMID: 13295598
[A method for fluorescence-optic demonstration of complement fixation and its use in the histo-immunological demonstration of experimental kidney anaphylaxis].
Dtsch Med Wochenschr. 1959 Nov 6;84:2001-4 PMID: 14409698
Staining of complement and modification of fluorescent antibody procedures.
J Immunol. 1958 Feb;80(2):122-31 PMID: 13514081

Article Info

Journal

The Journal of experimental medicine

Abbr.

J Exp Med

ISSN

0022-1007

DOI

10.1084/jem.111.1.93

Published

1960-01-01

Pages

93-106

Language

English

Region

United States

NLM ID

2985109R

PMCID

PMC2137196

Subset

External Links

PubMed source DOI: 10.1084/jem.111.1.93 PMC Full Text View journal details

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Studies on the antigenic properties of complement. I. Demonstration of agglutinating antibodies against guinea pig complement fixed on sensitized sheep erythrocytes.

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