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PMID: 1438229 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cloning and sequencing of a trophoblast-endothelial-activated lymphocyte surface protein: cDNA sequence and genomic structure.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 21 ·1992-11-01 ·Pages 10425-9

Voland JR, Wyzykowski RJ, Huang M, Dutton RW

Abstract

We have previously described the distribution of a surface glycoprotein recognized by monoclonal antibody B721. We now report the molecular characterization of this molecule at the protein, cDNA, and genomic level. A 75-kDa glycoprotein can be immunoprecipitated from B721+ tissues. We have isolated a near full-length cDNA containing the complete coding region and a full-length genomic clone. We present evidence that this protein has similarity to several classes of nuclear transcription factors, particularly the myc family of proteins. The 721P protein was found to have a leucine zipper-like structure, a possible basic region immediately upstream from the leucine zipper, and a protein kinase A phosphorylation site. 721P protein is encoded by a gene distinct from any deposited in existing data bases, and displays several features associated with proteins involved in signal transduction and gene regulation.

MeSH Terms

Amino Acid Sequence Animals Antibodies, Monoclonal Antigens Base Sequence Cell Line Chorionic Villi/physiology Cloning, Molecular/methods DNA/genetics,isolation & purification Endothelium, Vascular/physiology Female Gene Library Humans Leucine Zippers/genetics Membrane Glycoproteins/genetics Molecular Sequence Data Pregnancy Protein Conformation Proto-Oncogene Proteins c-myc/genetics Sequence Homology, Amino Acid Transcription Factors/genetics Transfection Umbilical Veins

Chemicals

Antibodies, Monoclonal Antigens Membrane Glycoproteins Proto-Oncogene Proteins c-myc Transcription Factors AKAP17A protein, human DNA

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Voland J R

Department of Biology and Cancer Center, University of California, San Diego, La Jolla 92093-0063.

Wyzykowski R J

Huang M

Dutton R W

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.89.21.10425

Published

1992-11-01

Pages

10425-9

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC50351

Subset

Grants

NIAID NIH HHS · AI 08795 · United States

NIAID NIH HHS · AI 23287 · United States

NIAID NIH HHS · AI 31870 · United States

Databases

GENBANK

M99578

External Links

PubMed source DOI: 10.1073/pnas.89.21.10425 PMC Full Text View journal details

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Cloning and sequencing of a trophoblast-endothelial-activated lymphocyte surface protein: cDNA sequence and genomic structure.

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