Antagonistic effects of 6-mercaptopurine and coenzyme A on mitochondria and mitosis in tissue culture.

The Journal of biophysical and biochemical cytology ·Vol. 1 ·No. 2 ·1955-03-00 ·Pages 119-26

BIESELE JJ

Abstract

The partial mitotic inhibition caused by 6-mercaptopurine in tissue cultures of Crocker mouse sarcoma 180 and embryonic mouse skin is blocked by co-enzyme A. 6-Mercaptopurine and coenzyme A also have opposite effects on mitochondrial morphology. Mitochondria in cells treated with 6-mercaptopurine become thin and fragmented. Coenzyme A blocks this effect, and alone coenzyme A makes for longer and thicker mitochondria. 6-Mercaptopurine inhibits lipogenesis in embryo skin fibroblasts, and this inhibition is partly counteracted by coenzyme A, which by itself makes for a greater accumulation of lipid droplets in the cytoplasm. It is suggested that at least one part of the action by which 6-mercaptopurine decreases mitotic incidence in tissue cultures may be an interference on the part of 6-mercaptopurine, acting as an antimetabolite of coenzyme A, in mitochondrial function related to cell division.

Keywords

CELL DIVISION COENZYMES MITOCHONDRIA PURINES/effects TISSUE CULTURE

MeSH Terms

Animals Antimetabolites Cell Division Coenzyme A Coenzymes Cytoplasm Fibroblasts Mercaptopurine Mice Mitochondria Mitosis Purines/pharmacology Sarcoma 180 Tissue Culture Techniques

Chemicals

Antimetabolites Coenzymes Purines Mercaptopurine Coenzyme A purine

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

BIESELE J J

References (27)

27 references, click to expand

Effects of purine antagonists on a diaminopurine-resistant strain of Lactobacillus casei.
J Biol Chem. 1953 Jun;202(2):647-54 PMID: 13061489
6-Mercaptopurine: effects in mouse sarcoma 180 and in normal animals.
Cancer Res. 1953 Aug;13(8):593-604 PMID: 13082494
Localization and integration of cellular function.
Cancer Res. 1953 Sep;13(9):617-32 PMID: 13094668
Clinical evaluation of a new antimetabolite, 6-mercaptopurine, in the treatment of leukemia and allied diseases.
Blood. 1953 Nov;8(11):965-99 PMID: 13105700
[Influence of coenzyme A on maintenance and conservation of tissues cultivated in vitro].
C R Seances Soc Biol Fil. 1953 Jun;147(11-12):978-82 PMID: 13116556
Studies on the fatty acid oxidizing system of animal tissues. III. Butyryl coenzyme A dehydrogenase.
J Biol Chem. 1954 Jan;206(1):1-12 PMID: 13130521
[Research on N-methyl-3,5,6-trihydroxyindole and adrenochrome in tissue culture. I. Action on growth and mitosis. II. Action on chondriome].
Arch Biol (Liege). 1953;64(4):399-437 PMID: 13139694
Studies on the fatty acid oxidizing system of animal tissues. V. Unsaturated fatty acyl coenzyme A hydrase.
J Biol Chem. 1954 Mar;207(1):125-32 PMID: 13152086
Enzymic mechanisms in the citric acid cycle.
Adv Enzymol Relat Subj Biochem. 1954;15:183-270 PMID: 13158180
Studies on the fatty acid oxidizing system of animal tissues. VI. beta-Hydroxyacyl coenzyme A dehydrogenase.
J Biol Chem. 1954 Apr;207(2):631-8 PMID: 13163047
Studies on the fatty acid oxidizing system of animal tissues. VII. The beta-ketoacyl coenzyme A cleavage enzyme.
J Biol Chem. 1954 May;208(1):345-57 PMID: 13174544
Development of the acetylation problem, a personal account.
Science. 1954 Nov 26;120(3126):855-65 PMID: 13216186
Action of various substances on the mitochondria of living cells cultivated in vitro.
Ann N Y Acad Sci. 1954 Nov 17;58(7):1246-63 PMID: 14350515
Microbiological effects of 6-mercaptopurine.
Ann N Y Acad Sci. 1954 Dec 6;60(2):200-6 PMID: 14350524
Biological activities of 6-mercaptopurine: effects on Streptococcus faecalis.
Ann N Y Acad Sci. 1954 Dec 6;60(2):212-9 PMID: 14350526
Effects of 6-mercaptopurine on experimental tumors in tissue culture.
Ann N Y Acad Sci. 1954 Dec 6;60(2):228-34 PMID: 14350528
Effects of 6-mercaptopurine and analogs on experimental tumors.
Ann N Y Acad Sci. 1954 Dec 6;60(2):235-43 PMID: 14350529
Studies on the toxicity and antileukemic action of 6-mercaptopurine in mice.
Ann N Y Acad Sci. 1954 Dec 6;60(2):251-66 PMID: 14350531
The toxic effects of 6-mercaptopurine and related compounds.
Ann N Y Acad Sci. 1954 Dec 6;60(2):283-96 PMID: 14350534
The fate of 6-mercaptopurine in mice.
Ann N Y Acad Sci. 1954 Dec 6;60(2):297-303 PMID: 14350535
The fate of 6-mercaptopurine in man.
Ann N Y Acad Sci. 1954 Dec 6;60(2):304-14 PMID: 14350536
On the mechanism of action of 6-mercaptopurine.
Ann N Y Acad Sci. 1954 Dec 6;60(2):315-21 PMID: 14350537
Levels and intracellular distribution of coenzyme A and pantothenic acid in rat liver and tumors.
Proc Soc Exp Biol Med. 1950 Nov;75(2):462-5 PMID: 14808293
Antagonists of nucleic acid derivatives. VI. Purines.
J Biol Chem. 1951 Oct;192(2):505-18 PMID: 14907641
The energy relations of mitotic activity in adult mouse epidermis.
Proc R Soc Lond B Biol Sci. 1951 Oct 30;138(893):562-75 PMID: 14911810
Enzymatic synthesis of citric acid by condensation of acetate and oxalacetate.
J Biol Chem. 1949 May;179(1):491 PMID: 18119265
Intracellular distribution of enzymes; the distribution of oxalacetic oxidase activity in rat liver and rat kidney fractions.
J Biol Chem. 1949 Feb;177(2):893-903 PMID: 18123817

Article Info

Journal

The Journal of biophysical and biochemical cytology

Abbr.

J Biophys Biochem Cytol

ISSN

0095-9901

DOI

10.1083/jcb.1.2.119

Published

1955-03-00

Pages

119-26

Language

English

Region

United States

NLM ID

17840020R

PMCID

PMC2223776

Subset

External Links

PubMed source DOI: 10.1083/jcb.1.2.119 PMC Full Text

Analysis Services

Antagonistic effects of 6-mercaptopurine and coenzyme A on mitochondria and mitosis in tissue culture.

Contact

WeChat Official Account