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PMID: 1435733 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Analysis of the site of action of the amdR product for regulation of the amdS gene of Aspergillus nidulans.

Molecular & general genetics : MGG ·Vol. 235 ·No. 1 ·1992-10-00 ·Pages 81-8

Littlejohn TG, Hynes MJ

Abstract

The amdR gene of Aspergillus nidulans regulates a number of structural genes in response to omega amino acid inducers. The site of action of the amdR product on expression of the amdS gene was investigated by studying the effects of changes in the 5' region of amdS, generated in vitro, on the induction, and on responses of an amdS-lacZ fusion gene to an amdRc allele. A sequence was identified that is sufficient for amdR regulation and that shows identity with sequences involved in amdR regulation of the gatA and lam genes. This sequence includes a CCAAT sequence and it was shown that this sequence is an important element in setting the basal level of amdS expression.

Related Genes
amdR amdS
MeSH Terms
Amidohydrolases/genetics Aspergillus nidulans/genetics Base Sequence Binding Sites DNA, Fungal Gene Expression Regulation, Enzymologic Gene Expression Regulation, Fungal Genes, Fungal Genes, Regulator Molecular Sequence Data Mutation Regulatory Sequences, Nucleic Acid Transformation, Genetic
Chemicals
DNA, Fungal Amidohydrolases acetamidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Littlejohn T G
Department of Genetics, University of Melbourne, Parkville, Victoria, Australia.
Hynes M J
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00286184
Published
1992-10-00
Pages
81-8
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00286184
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