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PMID: 14343125 Published · ppublish English Journal Article

THE ISOLATION AND CHARACTERIZATION OF SUBCELLULAR COMPONENTS OF THE EPITHELIAL CELLS OF RABBIT SMALL INTESTINE.

The Biochemical journal ·Vol. 96 ·1965-07-00 ·Pages 159-71

PORTEOUS JW, CLARK B

Abstract

1. Homogenization of the epithelial cells of rabbit small intestine in 0.3m-sucrose-5mm-EDTA, pH7.4, maintains intact the microvillus sheets that form the lumenal surface of the cells, the nuclei, the mitochondria and the vesicles (microsomes) formed from the endoplasmic reticulum. 2. These particulate components of the cell, and the cell-sap fraction, have been isolated by differential centrifuging of cell homogenates. 3. The nuclei and microvillus sheets sediment together and it has been impossible to separate these subcellular components by centrifugal methods. 4. The isolated subcellular fractions have been identified by a combination of light-microscopic examination, electron-microscopic examination, chemical analysis and assay for selected enzyme activities.

Keywords
ACID PHOSPHATASE ALKALINE PHOSPHATASE AMINOPEPTIDASES BIOCHEMISTRY CENTRIFUGATION CHOLESTEROL DNA EPITHELIUM EXPERIMENTAL LAB STUDY GLUCOSEPHOSPHATASE GLYCOSIDE HYDROLASES HOMOGENATES INTESTINAL MUCOSA INTESTINE SMALL MICROSCOPY ELECTRON RABBITS RNA SUCCINATE DEHYDROGENASE
MeSH Terms
Acid Phosphatase Alkaline Phosphatase Aminopeptidases Animals Biochemical Phenomena Biochemistry Centrifugation Cholesterol DNA Electrons Epithelial Cells Epithelium Glucose-6-Phosphatase Glycoside Hydrolases Intestinal Mucosa Intestine, Small Microscopy Microscopy, Electron RNA Rabbits Research Subcellular Fractions Succinate Dehydrogenase
Chemicals
RNA DNA Cholesterol Succinate Dehydrogenase Alkaline Phosphatase Acid Phosphatase Glucose-6-Phosphatase Glycoside Hydrolases Aminopeptidases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
PORTEOUS J W
CLARK B
References (46)
46 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj0960159
Published
1965-07-00
Pages
159-71
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1206917
Subset
OM
External Links
PubMed source DOI: 10.1042/bj0960159 PMC Full Text View journal details
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