THE BIOCHEMICAL CONVERSION OF 2-HYDROXY-4-METHYLTHIOBUTYRIC ACID INTO METHIONINE BY THE RAT IN VITRO.

The Biochemical journal ·Vol. 95 ·1965-06-00 ·Pages 683-7

LANER BW

Abstract

1. The conversion of 2-hydroxy-4-methylthiobarbituric acid into methionine requires a flavine coenzyme, which can be partially replaced by NAD or NADP, and glutamine, which can be partially replaced by asparagine. The system also requires aerobic conditions. 2. There does not appear to be a requirement for a metal ion: Mn(2+) is slightly inhibitory and Cu(2+) is strongly inhibitory. 3. Of the kidney, liver, brain, small intestine and muscle, the kidney possesses the greatest conversion activity/g. of tissue, but on a total organ basis the liver is the more important organ. Within the liver cells most of the activity is present in the supernatant fraction.

Keywords

AMINO ACID METABOLISM BRAIN COBALT COPPER EXPERIMENTAL LAB STUDY GLUTAMINE HYDROXYBUTYRATES INTESTINE SMALL IRON KIDNEY LIVER LIVER FUNCTION MAGNESIUM MANGANESE METHIONINE MOLYBDENUM MUSCLES PHARMACOLOGY RATS ZINC

MeSH Terms

Amino Acids/metabolism Brain Butyrates Calcium Cobalt Copper Glutamine Hydroxybutyrates In Vitro Techniques Intestine, Small Intestines Iron Kidney Liver/physiology Magnesium Manganese Methionine Molybdenum Muscles Pharmacology Rats Research Zinc

Chemicals

Amino Acids Butyrates Hydroxybutyrates Glutamine methylthiobutyric acid Cobalt Manganese Copper Molybdenum Methionine Iron Magnesium Zinc Calcium

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

LANER B W

References (2)

2 references, click to expand

Transamination from glutamine to alpha-keto acids.
J Biol Chem. 1950 Nov;187(1):173-87 PMID: 14794702
Transamination and associated deamidation of asparagine and glutamine.
J Biol Chem. 1952 May;197(1):319-30 PMID: 12981062

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj0950683

Published

1965-06-00

Pages

683-7

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1206794

Subset

External Links

PubMed source DOI: 10.1042/bj0950683 PMC Full Text View journal details

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THE BIOCHEMICAL CONVERSION OF 2-HYDROXY-4-METHYLTHIOBUTYRIC ACID INTO METHIONINE BY THE RAT IN VITRO.

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