Abstract
1. R-phycoerythrin from Ceramium rubrum and C-phycocyanin from Nostoc muscorum have been obtained in purified form by fractional crystallization, followed by chromatography and gel-filtration. 2. The amino acid composition of both chromoproteins was determined; 85% of the total weight of both was recovered as amino acids. 3. Alanine was identified as the only C-terminal amino acid of R-phycoerythrin, each molecule of which contained about 12 terminal groups. 4. Serine was identified as the only C-terminal group of C-phycocyanin.
Keywords
ALANINE
ALGAE
AMINO ACIDS
BIOCHEMISTRY
CHROMATOGRAPHY
CRYSTALLIZATION
EXPERIMENTAL LAB STUDY
GEL FILTRATION
PIGMENTS
SERINE
SPECTROPHOTOMETRY
MeSH Terms
Alanine
Amino Acids
Biochemical Phenomena
Biochemistry
Chromatography
Chromatography, Gel
Crystallization
Eukaryota
Phycocyanin
Phycoerythrin
Pigments, Biological
Proteins
Research
Serine
Spectrophotometry
Chemicals
Amino Acids
Pigments, Biological
Proteins
Phycocyanin
Phycoerythrin
Serine
Alanine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
RAFTERY M A
OHEOCHA C
References (11)
11 references, click to expand
-
Comparative studies of chromatographically separated phycoerythrins and phycocyanins.
Arch Biochem Biophys. 1955 Jan;54(1):162-73
PMID: 13229368
-
Studies on chromoproteins in Japanese nori, Porphyra tenera. V. On the sugar components of phycoerythrin.
J Biochem. 1961 May;49:361-7
PMID: 13702492
-
Gel filtration of proteins, peptides and amino acids.
Biochim Biophys Acta. 1960 Apr 8;39:193-207
PMID: 14434211
-
Chromatographic determination of cystine as cysteic acid.
Biochem J. 1954 May;57(1):33-7
PMID: 13159945
-
The molecular weights of phycoerythrin and phycocyan. I.
Biochem J. 1938 Mar;32(3):585-9
PMID: 16746657
-
The amino acid composition of crystalline pumpkin seed globulin, edestin, C-phycocyanin and R-phycoerythrin.
Bull Soc Chim Biol (Paris). 1958;40(12):2049-65
PMID: 13629245
-
The preparation and enzymatic hydrolysis of reduced and S-carboxymethylated proteins.
J Biol Chem. 1963 Feb;238:622-7
PMID: 14023808
-
STUDIES ON THE AMINO ACID SEQUENCE OF TOBACCO MOSAIC VIRUS PROTEIN. V. AMINO ACID SEQUENCES OF TWO PEPTIDES FROM TRYPTIC DIGESTS AND LOCATION OF AMIDE GROUP.
Arch Biochem Biophys. 1964 Apr;105:25-41
PMID: 14165501
-
Zone electrophoresis in starch gels: group variations in the serum proteins of normal human adults.
Biochem J. 1955 Dec;61(4):629-41
PMID: 13276348
-
A paper chromatographic method for the quantitative estimation of amino-acids.
Nature. 1954 Jul 17;174(4420):126-7
PMID: 13185263
-
The free amino groups of insulin.
Biochem J. 1945;39(5):507-15
PMID: 16747948