THE ADENOSINE-TRIPHOSPHATASE SYSTEM RESPONSIBLE FOR CATION TRANSPORT IN ELECTRIC ORGAN: EXCLUSION OF PHOSPHOLIPIDS AS INTERMEDIATES.

The Biochemical journal ·Vol. 94 ·1965-03-00 ·Pages 692-9

GLYNN IM, SLAYMAN CW, EICHBERG J, DAWSON RM

Abstract

1. Subcellular fractions were prepared from the electric organs of Electrophorus and Torpedo and assayed for adenosine-triphosphatase activity. 2. Treatment of the ;low-speed' fraction from Torpedo with m-urea gave an adenosine-triphosphatase preparation that was almost completely (98%) inhibited by ouabain (0.1mg./ml.) and dependent on the simultaneous presence of Na(+) and K(+). 3. The adenosine-triphosphatase preparations were exposed to [gamma-(32)P]ATP for 30sec. in the presence of (i) Na(+), (ii) K(+), (iii) Na(+)+K(+) and (iv) Na(+)+K(+)+ouabain. No significant labelling of phosphatidic acid, triphosphoinositide or any other phospholipid was observed. 4. The results suggest that phospholipids do not act as phosphorylated intermediates in the ;transport adenosine-triphosphatase' system of electric organ.

Keywords

ADENOSINE TRIPHOSPHATASE BIOLOGICAL TRANSPORT CELL MEMBRANE PERMEABILITY EELS ELECTRIC ORGAN ELECTRICITY EXPERIMENTAL LAB STUDY IONS METABOLISM MUSCLES OUABAIN PHARMACOLOGY PHOSPHOLIPIDS POTASSIUM SODIUM UREA

MeSH Terms

Adenosine Adenosine Triphosphatases Animals Biological Transport Cations Cell Membrane Permeability Eels Electric Organ Electricity Ions Metabolism Muscles Ouabain Pharmacology Phospholipids Potassium Research Sodium Urea

Chemicals

Cations Ions Phospholipids Ouabain Urea Sodium Adenosine Triphosphatases Adenosine Potassium

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

GLYNN I M

SLAYMAN C W

EICHBERG J

DAWSON R M

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25 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj0940692

Published

1965-03-00

Pages

692-9

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1206604

Subset

External Links

PubMed source DOI: 10.1042/bj0940692 PMC Full Text View journal details

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THE ADENOSINE-TRIPHOSPHATASE SYSTEM RESPONSIBLE FOR CATION TRANSPORT IN ELECTRIC ORGAN: EXCLUSION OF PHOSPHOLIPIDS AS INTERMEDIATES.

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