A STUDY ON THE GLUCOSAMINE-CONTAINING CONSTITUENTS OF THE SEEDS OF KIDNEY BEANS (PHASEOLUS VULGARIS).

The Biochemical journal ·Vol. 94 ·1965-03-00 ·Pages 604-10

PUSZTAI A

Abstract

1. The constituents of the seeds of kidney beans containing glucosamine that could be released by acid hydrolysis (0.5 n-hydrochloric acid at 100 degrees ) were extracted into the phenol-rich phase on partitioning between phenol and water at 70 degrees . 2. These materials were also brought into solution on extracting the seeds with water (incomplete), or preferably with a slightly alkaline buffer in the absence of phenol. When these solutions were heated at 100 degrees or treated with trichloroacetic acid, all the materials containing acid labile glucosamine were carried down with the precipitate. Treatment with pepsin rendered the materials containing glucosamine soluble again. 3. All proteins in these extracts of kidney bean were found to be associated with various amounts of neutral and amino sugars by high-voltage electrophoresis and chromatography on Sephadex G-200 columns. 4. The isolation and crystallization of the main sugar components, d-glucosamine and d-mannose, are described and their significance in plant glycoproteins is discussed.

Keywords

BIOCHEMISTRY CHROMATOGRAPHY ELECTROPHORESIS EXPERIMENTAL LAB STUDY GLUCOSAMINE GLYCOPROTEINS PLANT PROTEINS PLANTS EDIBLE SEEDS

MeSH Terms

Biochemical Phenomena Biochemistry Carbohydrates Chromatography Electrophoresis Glucosamine Glycoproteins Mannose Phaseolus Plant Proteins Plants Plants, Edible Research Seeds

Chemicals

Carbohydrates Glycoproteins Plant Proteins Glucosamine Mannose

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

PUSZTAI A

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj0940604

Published

1965-03-00

Pages

604-10

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1206594

Subset

External Links

PubMed source DOI: 10.1042/bj0940604 PMC Full Text View journal details

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A STUDY ON THE GLUCOSAMINE-CONTAINING CONSTITUENTS OF THE SEEDS OF KIDNEY BEANS (PHASEOLUS VULGARIS).

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