RAPID TECHNIQUE FOR THE ENUMERATION OF CLOSTRIDIUM PERFINGENS.

Applied microbiology ·Vol. 13 ·1965-07-00 ·Pages 559-63

MARSHALL RS, STEENBERGEN JF, MCCLUNG LS

Abstract

A new medium, Tryptone-sulfite-neomycin (TSN) agar, and an incubation procedure for the enumeration of Clostridium perfringens are described. Tolerance to neomycin, optimal growth at 46 C, and sulfite-reducing properties of C. perfringens were used as a basis for development of the medium. Comparisons were made between sulfite-polymyxin-sulfadiazine (SPS) agar and TSN agar at 37 and 46 C with C. perfringens and other organisms. These studies indicate the quantitative and selective superiority of TSN agar, incubated at 46 C, over SPS agar.

Keywords

CLASSIFICATION CLOSTRIDIUM CLOSTRIDIUM PERFRINGENS CULTURE MEDIA EXPERIMENTAL LAB STUDY NEOMYCIN PEPTONES PHARMACOLOGY PROTEUS SALMONELLA STREPTOCOCCUS SULFITES TEMPERATURE TRYPSIN

MeSH Terms

Agar Classification Clostridium Clostridium perfringens Culture Media Food Microbiology Neomycin Peptones Pharmacology Polymyxins Proteus Research Salmonella Streptococcus Sulfadiazine Sulfites Temperature Trypsin

Chemicals

Culture Media Peptones Polymyxins Sulfites Sulfadiazine Agar Trypsin Neomycin

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

MARSHALL R S

STEENBERGEN J F

MCCLUNG L S

References (10)

10 references, click to expand

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Rapid isolation of Clostridiums by selective inhibition of aerobic flora. I. Use of sorbic acid and polymyxin B sulfate in a liquid medium.
Am J Clin Pathol. 1956 Apr;26(4):418-21 PMID: 13313489
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Article Info

Journal

Applied microbiology

Abbr.

Appl Microbiol

ISSN

0003-6919

DOI

10.1128/am.13.4.559-563.1965

Published

1965-07-00

Pages

559-63

Language

English

Region

United States

NLM ID

7605802

PMCID

PMC1058297

Subset

External Links

PubMed source DOI: 10.1128/am.13.4.559-563.1965 PMC Full Text

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