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PMID: 1433518 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The requirements for viral entry differ from those for virally induced syncytium formation in NIH 3T3/DTras cells exposed to Moloney murine leukemia virus.

Journal of virology ·Vol. 66 ·No. 12 ·1992-12-00 ·Pages 7262-9

Wilson CA, Marsh JW, Eiden MV

Abstract

Moloney murine leukemia virus (Mo-MuLV) has the unique ability to infect different cells via either a low-pH-dependent or a pH-independent entry pathway. Only the pH-independent mechanism of Mo-MuLV entry has been associated with Mo-MuLV-induced syncytium formation. We have now identified a transformed cell line (NIH 3T3/DTras) which efficiently forms syncytia when exposed to Mo-MuLV, yet is low pH dependent for Mo-MuLV entry. Treatment of NIH 3T3/DTras cells with chloroquine, an agent which raises endosomal pH, blocks Mo-MuLV entry, but not Mo-MuLV-induced syncytium formation. This demonstrates that fusion which accompanies viral entry and fusion which is responsible for syncytium formation occur as independent processes in these cells. In addition, we determined that neither inherent differences in the Mo-MuLV receptor nor reduced affinity for Mo-MuLV gp70 can account for resistance of NIH 3T3 cells to Mo-MuLV-induced syncytium formation.

MeSH Terms
3T3 Cells Animals Cell Line Cell Transformation, Viral Chiroptera Chloroquine/pharmacology Giant Cells/cytology,physiology Humans Hydrogen-Ion Concentration Hylobates Membrane Fusion Mice Moloney murine leukemia virus/drug effects,physiology Rats Receptors, Virus/physiology
Chemicals
Receptors, Virus Chloroquine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wilson C A
Laboratory of Cell Biology, National Institute of Mental Health, Bethesda, Maryland 20892.
Marsh J W
Eiden M V
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.66.12.7262-7269.1992
Published
1992-12-00
Pages
7262-9
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC240430
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.66.12.7262-7269.1992 PMC Full Text View journal details
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