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PMID: 14333552 Published · ppublish English Journal Article

THE ESTIMATION OF THE OXIDIZED AND REDUCED FORMS OF THE NICOTINAMIDE NUCLEOTIDES.

The Biochemical journal ·Vol. 95 ·1965-04-00 ·Pages 161-6

GREENBAUM AL, CLARK JB, MCLEAN P

Abstract

1. A method is described for the determination of the oxidized and reduced forms of the nicotinamide nucleotides by measuring the rate of the oxygen uptake with an oxygen electrode in a system in which the nucleotide acts as the rate-limiting carrier in a cyclic system. 2. The method permits the measurement of quantities as low as 0.02mug. of NAD(+) or NADH or 0.01mug. of NADP(+) or NADPH. 3. The method permits the measurement of the nucleotides in extracts that contain non-specific reducing substances, coloured compounds or fluorescent materials, e.g. green leaves. 4. The results obtained by the present method are compared with those reported in the literature.

Keywords
ADIPOSE TISSUE ALCOHOL OXIDOREDUCTASES EXPERIMENTAL LAB STUDY GLUCOSEPHOSPHATE DEHYDROGENASE HEPATOMA LIVER MAMMAE NAD NADP PHARMACOLOGY PHENAZINES PLANTS EDIBLE POLAROGRAPHY RATS
MeSH Terms
Adipose Tissue Alcohol Oxidoreductases Animals Carcinoma, Hepatocellular Glucosephosphate Dehydrogenase Liver Liver Neoplasms Mammary Glands, Animal NAD NADP Niacinamide Nucleotides Oxidation-Reduction Pharmacology Phenazines Plants, Edible Polarography Rats Research
Chemicals
Nucleotides Phenazines NAD Niacinamide NADP Alcohol Oxidoreductases Glucosephosphate Dehydrogenase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
GREENBAUM A L
CLARK J B
MCLEAN P
References (19)
19 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj0950161
Published
1965-04-00
Pages
161-6
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1215190
Subset
OM
External Links
PubMed source DOI: 10.1042/bj0950161 PMC Full Text View journal details
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