ULTRASTRUCTURE OF A MYCOPLASMA (NEGRONI) ISOLATED FROM HUMAN LEUKEMIA.

Journal of bacteriology ·Vol. 90 ·1965-08-00 ·Pages 517-23

HUMMELER K, TOMASSINI N, HAYFLICK L

Abstract

Hummeler, Klaus (The Children's Hospital of Philadelphia, Philadelphia, Pa.), Natale Tomassini, and Leonard Hayflick. Ultrastructure of a mycoplasma (Negroni) isolated from human leukemia. J. Bacteriol. 90:517-523. 1965.-A strain of mycoplasma isolated in tissue cultures from human leukemic bone marrow was cultivated in cell-free media for 23 passages. Concentrates of broth cultures were investigated under the electron microscope in thin sections and negative contrast. The ultrastructure of the mature mycoplasma cells and their elementary bodies is described. It was found that the elementary bodies, particularly in the negative contrast preparations, bear a close resemblance to viruslike particles seen in tissues and plasmas of leukemic patients.

Keywords

BONE MARROW CELLS CULTURE MEDIA CYTOLOGY EXPERIMENTAL LAB STUDY LEUKEMIA MICROSCOPY ELECTRON MYCOPLASMA NEOPLASM ETIOLOGY TISSUE CULTURE

MeSH Terms

Bone Marrow Cells Cell Biology Culture Media Electrons Humans Leukemia Microscopy Microscopy, Electron Mycoplasma Neoplasms/etiology Research Tissue Culture Techniques

Chemicals

Culture Media

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

HUMMELER K

TOMASSINI N

HAYFLICK L

References (18)

18 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.90.2.517-523.1965

Published

1965-08-00

Pages

517-23

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC315673

Subset

External Links

PubMed source DOI: 10.1128/jb.90.2.517-523.1965 PMC Full Text View journal details

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