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PMID: 1430230 Published · ppublish English Journal Article

Retinoid protection against x-ray-induced chromatid damage in human peripheral blood lymphocytes.

The Journal of clinical investigation ·Vol. 90 ·No. 5 ·1992-11-00 ·Pages 2069-74

Sanford KK, Parshad R, Price FM, Tarone RE, Kraemer KH

Abstract

Oral administration of isotretinoin (13-cis retinoic acid) was shown previously (Kraemer, K. H., J. J. DiGiovanna, A. N. Moshell, R. E. Tarone, and G. L. Peck. 1988. N. Engl. J. Med. 318:1633-1637) to reduce the frequency of skin cancers in xeroderma pigmentosum (XP) patients. The mechanism of protection was unclear. In the present study, x-ray-induced chromatid damage in PHA-stimulated blood lymphocytes from five XP patients receiving isotretinoin was approximately half that in blood samples from the same patients before or subsequent to treatment. The x-ray-induced chromatid damage in blood lymphocytes from a normal control was reduced significantly by cocultivation with blood or plasma from an XP patient receiving isotretinoin or by addition of 10(-6) M isotretinoin to cultures 1 h before x-irradiation. A similar reduction in x-ray-induced chromatid damage was reported previously by adding to the culture medium, mannitol, a scavenger of the free hydroxyl radical, or catalase, which decomposes hydrogen peroxide; both of these products are generated during ionizing radiation. The present observations suggest that isotretinoin acts as a scavenger of such radiation products, thereby providing protection against x-ray-induced chromatid damage.

MeSH Terms
Adult Aged Cells, Cultured Chromatids/drug effects,radiation effects DNA Damage/drug effects Female Humans Isotretinoin/pharmacology Lymphocytes/drug effects,radiation effects,ultrastructure Male Middle Aged Skin Neoplasms/prevention & control X-Rays Xeroderma Pigmentosum/complications
Chemicals
Isotretinoin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Sanford K K
Laboratory of Cellular and Molecular Biology, National Cancer Institute, Bethesda, Maryland 20892.
Parshad R
Price F M
Tarone R E
Kraemer K H
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI116089
Published
1992-11-00
Pages
2069-74
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC443273
Subset
IM
Grants
Intramural NIH HHS · Z01 BC004517-31 · United States
External Links
PubMed source DOI: 10.1172/JCI116089 PMC Full Text View journal details
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