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PMID: 1429453 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Construction of an EcoRI restriction map of Mycoplasma pneumoniae and localization of selected genes.

Journal of bacteriology ·Vol. 174 ·No. 22 ·1992-11-00 ·Pages 7289-96

Wenzel R, Pirkl E, Herrmann R

Abstract

A restriction map of the genome of Mycoplasma pneumoniae, a small human pathogenic bacterium, was constructed by means of an ordered cosmid library which spans the complete bacterial chromosome. The positions of 143 endonuclease EcoRI restriction fragments were determined and aligned with the physical map. In addition, restriction sites for the rare-cutting enzymes XhoI (25 sites), ApaI (13 sites), NotI (2 sites), and SfiI (2 sites) were included. The resulting map consists of 185 restriction sites, has a mean resolution of 4.4 kbp, and predicts a genome size of 809 kbp. In addition, several genes were identified and mapped to their respective genomic EcoRI restriction fragments.

Related Genes
atpA deoC gyrA gyrB rplP rpsC rrn tuf
MeSH Terms
Amino Acid Sequence Base Sequence Blotting, Southern Cloning, Molecular Cosmids DNA, Bacterial/genetics,isolation & purification Deoxyribonuclease EcoRI/metabolism Genes, Bacterial Genome, Bacterial Macromolecular Substances Molecular Sequence Data Molecular Weight Mycoplasma pneumoniae/genetics Nucleic Acid Hybridization Oligodeoxyribonucleotides Proton-Translocating ATPases/genetics RNA, Transfer/genetics,isolation & purification Restriction Mapping Sequence Homology, Amino Acid
Chemicals
DNA, Bacterial Macromolecular Substances Oligodeoxyribonucleotides RNA, Transfer Deoxyribonuclease EcoRI Proton-Translocating ATPases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wenzel R
ZMBH, University of Heidelberg, Germany.
Pirkl E
Herrmann R
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.174.22.7289-7296.1992
Published
1992-11-00
Pages
7289-96
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC207423
Subset
IM
Databases
GENBANK
X67651, X67652, X67653
External Links
PubMed source DOI: 10.1128/jb.174.22.7289-7296.1992 PMC Full Text View journal details
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