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PMID: 1425586 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Laminin A chain: expression during Drosophila development and genomic sequence.

The EMBO journal ·Vol. 11 ·No. 12 ·1992-12-00 ·Pages 4519-27

Kusche-Gullberg M, Garrison K, MacKrell AJ, Fessler LI, Fessler JH

Abstract

A Drosophila laminin A chain gene was characterized as a 14 kb genomic nucleotide sequence which encodes an open reading frame of 3712 amino acids in 15 exons. Overall, this A chain is similar to its vertebrate counterparts, especially in its N- and C-terminal globular domains, but the sequence that forms the laminin A short arm is quite different and larger. Laminin messages appear in newly formed mesoderm and are later prominently expressed in hemocytes, which also synthesize basement membrane collagen IV. The composition of Drosophila basement membranes changes with development. A novel method of tandemly fused RNA probes showed that developmental increases of laminin mRNAs were primarily associated with periods of morphogenesis, and preceded those of collagen IV, a protein strongly expressed during growth. The ratio of A:B1:B2 mRNAs varied little during embryogenesis, with less mRNA for A than B chains. Staining of embryos with antibodies confirmed and extended the information provided by in situ hybridization. Homologs of the G-subdomains of this A chain, which occur in interacting regions of agrin, perlecan, laminin and sex steroid binding protein, may be involved in protein associations.

MeSH Terms
Amino Acid Sequence Animals Basement Membrane/metabolism Blotting, Northern Cell Line Collagen/metabolism Conserved Sequence Drosophila/embryology,genetics In Situ Hybridization Laminin/genetics,metabolism Larva Molecular Sequence Data RNA, Messenger/genetics Restriction Mapping
Chemicals
Laminin RNA, Messenger Collagen
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kusche-Gullberg M
Molecular Biology Institute, University of California, Los Angeles 90024-1570.
Garrison K
MacKrell A J
Fessler L I
Fessler J H
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1992-12-00
Pages
4519-27
Language
English
Region
England
NLM ID
8208664
PMCID
PMC557027
Subset
IM
Grants
NIA NIH HHS · AG02128 · United States
NIGMS NIH HHS · GM07185 · United States
Databases
GENBANK
M75882, M94777, M94778, M94779, M94780, M94781, M94782, M94783, M94784, M94785, M96388
External Links
PubMed source PMC Full Text View journal details
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