EXPERIMENTAL SALMONELLOSIS. IV. LIPID CONTENT OF TOXIN L OBTAINED FROM SALMONELLA ENTERITIDIS.

Journal of bacteriology ·Vol. 89 ·1965-01-00 ·Pages 193-7

KAWAKAMI M, MITSUHASHI S

Abstract

Kawakami, Masaya (Gunma University, Maebashi, Japan), and Susumu Mitsuhashi. Experimental salmonellosis. IV. Lipid content of toxin L obtained from Salmonella enteritidis. J. Bacteriol. 89:193-197. 1965.-An improved method is described for the chromatographic purification of toxin L from virulent Salmonella enteritidis. Toxin L is heat-labile, and its toxicity is reduced by trypsin and by phospholipase A treatment. Chemical studies of chromatographically and ultracentrifugally homogeneous toxin preparations show that toxin L is a phosphorus-containing protein. The infrared-absorption spectrum of a petroleum ether extract of toxin L hydrolysate indicates that this material possesses a lipid component.

Keywords

CHROMATOGRAPHY EXPERIMENTAL LAB STUDY LIPIDS PHOSPHOLIPASE SALMONELLA SPECTROPHOTOMETRY TOXICOLOGIC REPORT TOXINS AND ANTITOXINS TRYPSIN ULTRACENTRIFUGATION

MeSH Terms

Antitoxins Chromatography Japan Lipids Phospholipases Research Salmonella Salmonella Infections Salmonella enteritidis Spectrophotometry Toxicology Toxins, Biological Trypsin Ultracentrifugation

Chemicals

Antitoxins Lipids Toxins, Biological Phospholipases Trypsin

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

KAWAKAMI M

MITSUHASHI S

References (10)

10 references, click to expand

Studies on Habu snake venom. (V). Myolysis caused by phospholipase A in Habu snake venom.
Jpn J Exp Med. 1962 Feb;32:55-64 PMID: 14468184
[THE PRESENT STAGE OF RESEARCH ON THE NEUROTOXINS OF GRAM-NEGATIVE BACTERIA].
Arch Roum Pathol Exp Microbiol. 1963 Sep;23:775-82 PMID: 14165860
Immunochemical study of a bacterial endotoxin: Shigella flexneri type Z.
J Exp Med. 1954 Oct 1;100(4):391-404 PMID: 13201716
Quantitative Determination of Carbohydrates With Dreywood's Anthrone Reagent.
Science. 1948 Mar 5;107(2775):254-5 PMID: 17814729
EXPERIMENTAL SALMONELLOSIS. III. NEW TOXIC FRACTION (L) OBTAINED FROM SALMONELLA ENTERITIDIS AND ITS IMMUNOLOGICAL PROPERTIES.
J Bacteriol. 1963 Oct;86:872-9 PMID: 14066488
On the nature of the toxic component of the somatic antigen of Shigella paradysenteriae type Z (Flexner).
J Exp Med. 1950 Jul 1;92(1):25-34 PMID: 15422094
Procedures for the chromatographic determination of amino acids on four per cent cross-linked sulfonated polystyrene resins.
J Biol Chem. 1954 Dec;211(2):893-906 PMID: 13221595
[Complex formation of bacterial lipopolysaccharides with proteins and lipids].
Biochem Z. 1958;330(1):21-33 PMID: 13535613
Studies in immunochemistry: The use of phenol and of alkali in the degradation of antigenic material isolated from Bact. dysenteriae (Shiga).
Biochem J. 1941 Nov;35(10-11):1140-63 PMID: 16747399
The estimation of phosphorus.
Biochem J. 1940 Jun;34(6):858-65 PMID: 16747230

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.89.1.193-197.1965

Published

1965-01-00

Pages

193-7

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC315568

Subset

External Links

PubMed source DOI: 10.1128/jb.89.1.193-197.1965 PMC Full Text View journal details

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EXPERIMENTAL SALMONELLOSIS. IV. LIPID CONTENT OF TOXIN L OBTAINED FROM SALMONELLA ENTERITIDIS.

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