The appositional region between the intermediate element and adjacent cisternae in the triads of the sarcoplasmic reticulum of striated muscle from humans, copepods, ostracods, and barnacles shows a five-layered construction similar to that of a tight junction. Known functions of tight junctions and cisternal elements suggest that a membrane depolarization, conducted by the intermediate element, is transmitted to the cisternae by way of the triadic junction to cause a release of calcium ions from the cisternae.
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