5-bromo-2'-deoxyuridine-stimulated calcium ion- or magnesium ion-dependent ecto-(adenosine triphosphatase) activity of cultured hamster cardiac cells.

The Biochemical journal ·Vol. 164 ·No. 3 ·1977-06-15 ·Pages 645-52

Coetzee GA, Gevers W

Abstract

1. Treatment of hamster heart cells in primary culture with 5-bromo-2'-deoxyuridine resulted in the greatly increased activity of a particulate Ca2+- or Mg2+-dependent ATPase (adenosine triphosphatase). 2. 5-Bromo-2'-deoxyuridine exerted these effects only when it was incorporated into cellular DNA, and then in a concentration-dependent manner. 3. Serially replated cells contained less of the activity (expressed as a function of total cell protein) than did the primary cultures, but the stimulation caused by 5-bromo-2'-deoxyuridine addition was much greater. 4. The affected enzyme was apparently localized in the plasma membrane of the cells with its active centre exposed to the outer environment [ecto-(ATPase) dependent on Ca2+ or Mg2+].5. The activity was unaffected by treatment with p-chloromercuriphenylsulphonate, ouabain andverapamil. 6. Ecto (5'-nucleotidase) activity was not increased by 5-bromo-2'-deoxyuridine treatment of cells, and ecto-(p-nitrophenyl phosphatase) activity was only slightly enhanced.

MeSH Terms

Adenosine Triphosphatases/metabolism Animals Bromodeoxyuridine/pharmacology Calcium Cells, Cultured Cricetinae DNA/metabolism Heart/drug effects Magnesium Mesocricetus Myocardium/cytology,enzymology

Chemicals

DNA Adenosine Triphosphatases Bromodeoxyuridine Magnesium Calcium

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Coetzee G A

Gevers W

References (32)

32 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1640645

Published

1977-06-15

Pages

645-52

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1164842

Subset

External Links

PubMed source DOI: 10.1042/bj1640645 PMC Full Text View journal details

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5-bromo-2'-deoxyuridine-stimulated calcium ion- or magnesium ion-dependent ecto-(adenosine triphosphatase) activity of cultured hamster cardiac cells.

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