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PMID: 1421580 Published · ppublish English Journal Article

Signal transduction by the platelet integrin alpha IIb beta 3: induction of calcium oscillations required for protein-tyrosine phosphorylation and ligand-induced spreading of stably transfected cells.

Molecular biology of the cell ·Vol. 3 ·No. 9 ·1992-09-00 ·Pages 989-98

Pelletier AJ, Bodary SC, Levinson AD

Abstract

We demonstrate an example of signal transduction by an integrin and have begun to define the pathway through which this signaling is achieved. We constructed a stably transfected derivative of 293 cells (ATCC 1573) that expresses the platelet integrin GPIIbIIIa (alpha IIb beta 3). This cell line, clone B, adheres to and spreads on fibrinogen, a ligand for alpha IIb beta 3, while the parent cell line does not. Stimulation of these cells either by adhesion to fibrinogen or with antiserum directed against alpha IIb beta 3 results in induction of calcium oscillations, followed by tyrosine phosphorylation of at least one protein of molecular weight approximately 125 kDa. We establish that this phosphorylation, as well as the morphological rearrangements, requires the mobilization of calcium.

MeSH Terms
Blood Platelets/metabolism Calcium/metabolism Cell Adhesion Cell Line Cell Size Fibrinogen Integrins/metabolism Periodicity Phosphorylation Signal Transduction Transfection Tyrosine/metabolism
Chemicals
Integrins Tyrosine Fibrinogen Calcium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Pelletier A J
Genentech Inc., South San Francisco, California 94080.
Bodary S C
Levinson A D
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.3.9.989
Published
1992-09-00
Pages
989-98
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC275660
Subset
IM
External Links
PubMed source DOI: 10.1091/mbc.3.9.989 PMC Full Text View journal details
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