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PMID: 14212122 Published · ppublish English Journal Article

EQUINE ANTIHAPTEN ANTIBODY. I. 7S BETA-2A- AND 1OS GAMMA-1- GLOBULIN COMPONENTS OF PURIFIED ANTI-BETA-LACTOSIDE ANTIBODY.

The Journal of experimental medicine ·Vol. 120 ·1964-10-01 ·Pages 589-609

ROCKEY JH, KLINMAN NR, KARUSH F

Abstract

Anti-p-azophenyl-beta-lactoside (Lac) antibody has been isolated from equine antiserum by specific precipitation with Lac-HSA (human serum albumin) and Lac-Hy (hemocyanin). Antibody was separated from antigen by chromatography on DEAE cellulose and zone electrophoresis in solvents containing lactose. Six antigenically distinct immunoglobulins have been identified in purified equine anti-Lac antibody: 7S beta(2A)-globulin, 19S gamma-globulin, a 10S gamma(1) globulin, and three antigenically distinct 7S gamma-globulins. The specific hapten-binding activity of the 7S beta(2A)-antibody and of the 10S gamma(1)-antibody has been demonstrated by equilibrium dialysis and by analytical ultracentrifugation using a combination of schlieren and absorption optics. The 10S gamma(1)-globulin antibody may be representative of a hitherto undescribed class of immunoglobulins.

Keywords
ANTIBODIES BETA GLOBULIN CHROMATOGRAPHY EXPERIMENTAL LAB STUDY GAMMA GLOBULIN 7S HAPTENS HORSES IMMUNOELECTROPHORESIS LACTOSE SERUM ALBUMIN ULTRACENTRIFUGATION
MeSH Terms
Animals Antibodies Antigens Beta-Globulins Chromatography Glycosides Haptens Hemocyanins Horses Immune Sera Immunoelectrophoresis Immunoglobulin G Immunoglobulin M Lactose Research Serum Albumin Ultracentrifugation gamma-Globulins
Chemicals
Antibodies Antigens Beta-Globulins Glycosides Haptens Immune Sera Immunoglobulin G Immunoglobulin M Serum Albumin gamma-Globulins lactosides Hemocyanins Lactose
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
ROCKEY J H
KLINMAN N R
KARUSH F
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31 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.120.4.589
Published
1964-10-01
Pages
589-609
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2137781
Subset
OM
External Links
PubMed source DOI: 10.1084/jem.120.4.589 PMC Full Text View journal details
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