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PMID: 1421152 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Isolation and characterization of a tobacco gene with homology to pectate lyase which is specifically expressed during microsporogenesis.

Plant molecular biology ·Vol. 20 ·No. 3 ·1992-11-00 ·Pages 493-502

Rogers HJ, Harvey A, Lonsdale DM

Abstract

A genomic clone has been isolated which contains an open reading frame of 1191 bp interrupted by two small introns. The ORF has been sequenced and the transcriptional start determined. The predicted amino acid sequence shows homology to the deduced amino acid sequences of two pollen-specific pectate lyase genes identified in tomato. The genomic clone was isolated using a partial cDNA clone, TP10, which had been isolated from a Nicotiana tabacum pollen cDNA library by means of differential screening. TP10 has been fully sequenced and contains an open reading frame of 792 bp which shows 96% homology to the ORF in the genomic clone. The transcript corresponding to TP10 is maximally expressed late in pollen development, and has not been detected in vegetative tissues.

MeSH Terms
Amino Acid Sequence Base Sequence Chromosome Mapping Gene Library Genes, Plant/genetics Molecular Sequence Data Morphogenesis/genetics Open Reading Frames Plants, Toxic Pollen/genetics Polysaccharide-Lyases/genetics RNA, Messenger/genetics Sequence Homology, Amino Acid Tissue Distribution Tobacco/enzymology,genetics,growth & development Transcription, Genetic
Chemicals
RNA, Messenger Polysaccharide-Lyases pectate lyase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rogers H J
Cambridge Laboratory, AFRC Institute of Plant Science Research, John Innes Centre, Norwich, UK.
Harvey A
Lonsdale D M
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29 references, click to expand
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/BF00040608
Published
1992-11-00
Pages
493-502
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
Databases
GENBANK
X67158, X67159
External Links
PubMed source DOI: 10.1007/BF00040608 View journal details
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