PRODUCTION OF ANTISERUM FOR STAPHYLOCOCCAL ENTEROTOXIN.

Applied microbiology ·Vol. 12 ·1964-07-00 ·Pages 363-7

CASMAN EP, BENNETT RW

Abstract

Immunization of rabbits with approximately 95% pure enterotoxin B and approximately 20 and 50% pure enterotoxin A yielded specific antisera which required no and little absorption, respectively, when used in the slide double-diffusion test. Methods for purifying enterotoxin A and for the production and absorption of antisera are presented.

Keywords

ELECTROPHORESIS ENTEROTOXINS EXPERIMENTAL LAB STUDY GEL DIFFUSION TESTS IMMUNE SERUMS PRECIPITIN TESTS RABBITS STAPHYLOCOCCUS

MeSH Terms

Animals Electrophoresis Enterotoxins Immune Sera Immunodiffusion Lagomorpha Precipitin Tests Rabbits Research Staphylococcus

Chemicals

Enterotoxins Immune Sera

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

CASMAN E P

BENNETT R W

References (10)

10 references, click to expand

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Acta Pathol Microbiol Scand. 1953;32(2):230-40 PMID: 13079779
Studies on immunity to toxins of Clostridium botulinum. III. Preparation, purification, and detoxification of type E toxin.
J Bacteriol. 1957 Oct;74(4):533-8 PMID: 13475277
A slide microtechnique for the analysis of immune precipitates in gel.
Int Arch Allergy Appl Immunol. 1957;10(6):355-60 PMID: 13501906
Serologic studies of staphylococcal enterotoxin.
Public Health Rep. 1958 Jul;73(7):599-609 PMID: 13568011
A simplified micro double-diffusion agar precipitin technique.
J Lab Clin Med. 1958 Nov;52(5):784-7 PMID: 13588200
DESIGNATION OF STAPHYLOCOCCAL EXTEROTOXINS.
J Bacteriol. 1963 Mar;85:715-6 PMID: 14042955
CULTURE MEDIUM FOR THE PRODUCTION OF STAPHYLOCOCCAL ENTEROTOXIN A.
J Bacteriol. 1963 Jul;86:18-23 PMID: 14051816
Staphylococcal enterotoxin. I. Purification.
Arch Biochem Biophys. 1959 Nov;85:62-9 PMID: 13799261
Further serological studies of staphylococcal enterotoxin.
J Bacteriol. 1960 Jun;79:849-56 PMID: 13808165
A Method for the Aeration of Liquid Cultures of Microorganisms.
Science. 1948 Apr 9;107(2780):377-8 PMID: 17783092

Article Info

Journal

Applied microbiology

Abbr.

Appl Microbiol

ISSN

0003-6919

DOI

10.1128/am.12.4.363-367.1964

Published

1964-07-00

Pages

363-7

Language

English

Region

United States

NLM ID

7605802

PMCID

PMC1058134

Subset

External Links

PubMed source DOI: 10.1128/am.12.4.363-367.1964 PMC Full Text

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PRODUCTION OF ANTISERUM FOR STAPHYLOCOCCAL ENTEROTOXIN.

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