PURIFICATION AND MOLECULAR WEIGHT DETERMINATION OF CLOSTRIDIUM BOTULINUM TYPE E TOXIN.

Journal of bacteriology ·Vol. 88 ·1964-07-00 ·Pages 216-9

GERWING J, DOLMAN CE, REICHMANN ME, BAINS HS

Abstract

Gerwing, Julia (The University of British Columbia, Vancouver, British Columbia, Canada), Claude E. Dolman, M. E. Reichmann, and Hardial S. Bains. Purification and molecular weight determination of Clostridium botulinum type E toxin. J. Bacteriol. 88:216-219. 1964.-A method was developed whereby type E botulinus toxin can be obtained in a highly purified state by elution through acidified diethylaminoethyl-cellulose columns. The material thus isolated appears to be electrophoretically and ultracentrifugally homogeneous. A molecular weight of 18,600 was calculated for the toxin.

Keywords

CHEMISTRY ANALYTICAL CHROMATOGRAPHY CLOSTRIDIUM BOTULINUM ELECTROPHORESIS EXPERIMENTAL LAB STUDY MOLECULAR WEIGHT TOXINS AND ANTITOXINS ULTRACENTRIFUGATION

MeSH Terms

Antitoxins Botulinum Toxins British Columbia Canada Chemistry Techniques, Analytical Chromatography Clostridium botulinum Clostridium botulinum type E Electrophoresis Molecular Weight Research Toxins, Biological Ultracentrifugation

Chemicals

Antitoxins Toxins, Biological Botulinum Toxins botulinum toxin type E

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

GERWING J

DOLMAN C E

REICHMANN M E

BAINS H S

References (11)

11 references, click to expand

Purification and activation of Clostridium botulinum type E toxin.
J Bacteriol. 1961 May;81:819-22 PMID: 13704800
Activation of Clostridium botulinum type E toxin by trypsin.
J Bacteriol. 1956 Oct;72 (4):455-60 PMID: 13366946
Activation phenomenon of Clostridium botulinum type E toxin.
J Bacteriol. 1962 Aug;84:302-6 PMID: 13898102
Isolation and sedimentation study of low molecular weight forms of type A Botulins toxin.
Arch Biochem Biophys. 1954 May;50(1):104-12 PMID: 13159305
Studies on the toxin production of Clostridium botulinum type E. I. A strain of genus Clostridium having the action to promote type E botulinal toxin production in a mixed culture.
Jpn J Med Sci Biol. 1955 Jun;8(3):247-53 PMID: 13285961
The Purification and Crystallization of Clostridium botulinum Type A Toxin.
Science. 1946 May 17;103(2681):613-4 PMID: 17808402
Studies on toxin production of Clostridium botulinum type E. III. Characterization of toxin precursor.
J Bacteriol. 1959 Jul;78(1):1-9 PMID: 13672902
The Isolation of Type B Botulinum Toxin.
J Bacteriol. 1947 Nov;54(5):575-84 PMID: 16561395
Studies on immunity to toxins of Clostridium botulinum. III. Preparation, purification, and detoxification of type E toxin.
J Bacteriol. 1957 Oct;74(4):533-8 PMID: 13475277
The behavior of the bolulinus toxins in the ultracentrifuge.
Arch Biochem Biophys. 1951 May;31(3):424-30 PMID: 14830257
Recent observations on type E botulism.
Can J Public Health. 1957 May;48(5):187-98 PMID: 13426860

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.88.1.216-219.1964

Published

1964-07-00

Pages

216-9

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC277279

Subset

External Links

PubMed source DOI: 10.1128/jb.88.1.216-219.1964 PMC Full Text View journal details

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