TYPING OF GROUP A STREPTOCOCCI BY IMMUNOFLUORESCENCE. I. PREPARATION AND PROPERTIES OF TYPE I FLUORESCEIN-LABELED ANTIBODY.

Journal of bacteriology ·Vol. 87 ·1964-06-00 ·Pages 1377-82

KARAKAWA WW, BORMAN EK, MCFARLAND CR

Abstract

Karakawa, Walter W. (Connecticut State Department of Health, Hartford), Earle K. Borman, and Clarence R. McFarland. Typing of group A streptococci by immunofluorescence. I. Preparation and properties of type 1 fluorescein-labeled antibody. J. Bacteriol. 87:1377-1382. 1964.-Unadsorbed, fluorescein-labeled globulins derived from rabbits immunized with acid-extracted M-protein of type 1 streptococci (plus adjuvant) were found to have high fluorescent-antibody (FA) staining titers and to be considerably more type-specific than were similar preparations derived from whole-cell immunization. Appropriate adsorption rendered the anti-M reagent entirely type-specific without appreciable loss of titer; whole-cell reagent was appreciably weakened in FA titer by comparable adsorption. Type-specificity was confirmed by parallel bactericidal, long-chain, and precipitin studies. Removal of reactivity by adsorption with homologous M protein was complete, confirming that the FA reaction was truly a manifestation of an M anti-M protein system. The data indicate that the development of FA reagents specific for the streptococcal types is feasible.

Keywords

BACTERIOLOGICAL TECHNICS EXPERIMENTAL LAB STUDY FLUORESCENT ANTIBODY TECHNIC STREPTOCOCCUS

MeSH Terms

Animals Bacteriological Techniques Fluorescein Fluorescent Antibody Technique Rabbits Research Staining and Labeling Streptococcus Streptococcus pyogenes

Chemicals

Fluorescein

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

KARAKAWA W W

BORMAN E K

MCFARLAND C R

References (10)

10 references, click to expand

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Superiority of fluorescein isothiocyanate (Riggs) for fluorescent-antibody technic with a modification of its application.
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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.87.6.1377-1382.1964

Published

1964-06-00

Pages

1377-82

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC277213

Subset

External Links

PubMed source DOI: 10.1128/jb.87.6.1377-1382.1964 PMC Full Text View journal details

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TYPING OF GROUP A STREPTOCOCCI BY IMMUNOFLUORESCENCE. I. PREPARATION AND PROPERTIES OF TYPE I FLUORESCEIN-LABELED ANTIBODY.

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