CHROMOSOMAL LOCATION OF THYMINE AND ARGININE GENES IN ESCHERICHIA COLI AND AN F' INCORPORATING THEM.

Journal of bacteriology ·Vol. 87 ·1964-03-00 ·Pages 554-61

ISHIBASHI M, SUGINO Y, HIROTA Y

Abstract

Ishibashi, Masahide (Osaka University, Osaka, Japan), Yoshinobu Sugino, and Yukinori Hirota. Chromosomal location of thymine and arginine genes in Escherichia coli and an F' incorporating them. J. Bacteriol. 87:554-561. 1964.-The gene responsible for thymine requirement or independence in Escherichia coli K-12 is located on the chromosome, near Arg(2) (arginine) and between Sm (streptomycin) and Ade (adenine). A new F' (called F(15)) carrying the Thy (thymine) gene as well as the Arg(2) gene was discovered. The Thy(+) (thymine independent) gene on F(15) relieves the defect of all Thy(-) (thymine requiring) mutants of E. coli examined, such as E. coli 15 T(-), B3, and Thy(-) mutants obtained by aminopterine treatment. It can also be transferred to Thy(-) mutants of Salmonella typhimurium and Serratia marcescens, converting them to Thy(+).

Keywords

ADENINE AMINOPTERIN ARGININE CHROMOSOMES ESCHERICHIA COLI EXPERIMENTAL LAB STUDY HISTIDINE LEUCINE METHIONINE MUTATION NICOTINIC ACID PURINES THREONINE THYMINE TRYPTOPHAN

MeSH Terms

Adenine Aminopterin Arginine Chromosomes Escherichia coli Escherichia coli K12 Histidine Japan Leucine Methionine Mutation Niacin Nicotinic Acids Purines Research Streptomycin Threonine Thymine Tryptophan

Chemicals

Nicotinic Acids Purines Niacin Threonine Histidine Tryptophan Arginine Methionine Leucine Adenine Aminopterin Thymine purine Streptomycin

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

ISHIBASHI M

SUGINO Y

HIROTA Y

References (13)

13 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.87.3.554-561.1964

Published

1964-03-00

Pages

554-61

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC277053

Subset

External Links

PubMed source DOI: 10.1128/jb.87.3.554-561.1964 PMC Full Text View journal details

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CHROMOSOMAL LOCATION OF THYMINE AND ARGININE GENES IN ESCHERICHIA COLI AND AN F' INCORPORATING THEM.

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