THIN-LAYER CHROMATOGRAPHY AND BIOASSAY OF PROSTAGLANDINS IN EXTRACTS OF SEMEN AND TISSUES OF THE MALE REPRODUCTIVE TRACT.

British journal of pharmacology and chemotherapy ·Vol. 22 ·1964-02-00 ·Pages 183-8

HORTON EW, THOMPSON CJ

Abstract

By acid ether extraction and thin-layer chromatography, prostaglandins have been separated from biologically active compounds of other chemical groups. The technique does not, however, separate different prostaglandins from each other. The biological activity of the eluates was estimated on the rabbit isolated jejunum and the hamster isolated colon preparations in terms of prostaglandin E(1); the concentrations (thus expressed) of prostaglandin in human semen ranged from 24 to 783 mug/ml. with a mean of 226 mug/ml. No prostaglandins (minimal detectable concentration, 0.5 mug/g) could be detected in the male reproductive organs of several species of laboratory animal.

Keywords

BIOLOGICAL ASSAY BIOLOGICAL PRODUCTS CATS CHROMATOGRAPHY COLON EXPERIMENTAL LAB STUDY GUINEA PIGS HAMSTERS JEJUNUM MICE PENIS PROSTAGLANDINS PROSTATE RABBITS RATS SEMEN SEMINAL VESICLES TESTIS TISSUE EXTRACTS

MeSH Terms

Animals Biological Assay Biological Products Cats Chromatography Chromatography, Thin Layer Colon Cricetinae Guinea Pigs Humans Jejunum Lagomorpha Male Mice Penis Prostaglandins Prostaglandins E Prostate Rabbits Rats Research Semen Seminal Vesicles Testis Tissue Extracts

Chemicals

Biological Products Prostaglandins Prostaglandins E Tissue Extracts

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

HORTON E W

THOMPSON C J

References (6)

6 references, click to expand

Some biological effects of two crystalline prostaglandin factors.
Acta Physiol Scand. 1959 Mar 31;45:133-44 PMID: 13799460
On the specific vaso-dilating and plain muscle stimulating substances from accessory genital glands in man and certain animals (prostaglandin and vesiglandin).
J Physiol. 1936 Nov 6;88(2):213-34 PMID: 16994817
Semen prostaglandin levels in fifty patients attending a fertility clinic.
J Reprod Fertil. 1961 Feb;2:1-10 PMID: 13712407
A COMPARISON OF THE BIOLOGICAL ACTIVITIES OF FOUR PROSTAGLANDINS.
Br J Pharmacol Chemother. 1963 Aug;21:182-9 PMID: 14066143
ISOLATION AND IDENTIFICATION OF TWO SMOOTH MUSCLE STIMULANTS FROM MENSTRUAL FLUID.
Nature. 1963 Dec 7;200:960 PASSIM PMID: 14097744
Isolation of prostaglandin E1 from human seminal plasma. Prostaglandins and related factors. 11.
J Biol Chem. 1962 Sep;237:3005-6 PMID: 13867832

Article Info

Journal

British journal of pharmacology and chemotherapy

Abbr.

Br J Pharmacol Chemother

ISSN

0366-0826

DOI

10.1111/j.1476-5381.1964.tb01557.x

Published

1964-02-00

Pages

183-8

Language

English

Region

England

NLM ID

0154627

PMCID

PMC1703900

Subset

External Links

PubMed source DOI: 10.1111/j.1476-5381.1964.tb01557.x PMC Full Text

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THIN-LAYER CHROMATOGRAPHY AND BIOASSAY OF PROSTAGLANDINS IN EXTRACTS OF SEMEN AND TISSUES OF THE MALE REPRODUCTIVE TRACT.

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