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PMID: 1409714 Published · ppublish English Journal Article

Genetically engineered rice resistant to rice stripe virus, an insect-transmitted virus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 20 ·1992-10-15 ·Pages 9865-9

Hayakawa T, Zhu Y, Itoh K, Kimura Y, Izawa T, Shimamoto K, Toriyama S

Abstract

The coat protein (CP) gene of rice stripe virus was introduced into two japonica varieties of rice by electroporation of protoplasts. The resultant transgenic plants expressed the CP at high levels (up to 0.5% of total soluble protein) and exhibited a significant level of resistance to virus infection. Plants derived from selfed progeny of the primary transformants also expressed the CP and showed viral resistance, indicating stable transmission of the CP gene and the trait of resistance to the next generation. Moreover, the virally encoded strip disease-specific protein was not detected in transgenic plants expressing CP 8 weeks after inoculation, indicating protection before viral multiplication. These studies demonstrated that CP-mediated resistance to virus infection can be extended to cereals and to the viruses transmitted by an insect vector (planthopper).

MeSH Terms
Base Sequence Capsid/genetics Gene Expression Immunity, Innate/genetics Molecular Sequence Data Oligodeoxyribonucleotides/chemistry Oryza/genetics,microbiology Plant Diseases Plant Viruses/genetics,growth & development Plants, Genetically Modified Polymerase Chain Reaction RNA, Messenger/genetics Virus Diseases/prevention & control Virus Replication
Chemicals
Oligodeoxyribonucleotides RNA, Messenger
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Hayakawa T
Plantech Research Institute, Yokohama, Japan.
Zhu Y
Itoh K
Kimura Y
Izawa T
Shimamoto K
Toriyama S
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.20.9865
Published
1992-10-15
Pages
9865-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC50234
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.89.20.9865 PMC Full Text View journal details
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