THE BASE COMPOSITION OF RIBONUCLEIC ACID IN LAMPBRUSH CHROMOSOMES, NUCLEOLI, NUCLEAR SAP, AND CYTOPLASM OF TRITURUS OOCYTES.

The Journal of cell biology ·Vol. 19 ·1963-11-00 ·Pages 279-84

EDSTROEM JE, GALL JG

Abstract

The base composition of RNA's extracted from chromosomes, nucleoli, nuclear sap, and cytoplasm of Triturus oocytes has been determined by microelectrophoresis. The chromosomal RNA has a content of guanine+cytosine equal to that of DNA, but there is no complementarity in the composition as for DNA. Nuclear sap contains a highly variable RNA with a tendency towards high uracil values. Nucleolar and cytoplasmic RNA's are similar in composition and both are of the guanine-cytosine rich type. The chromosomes and nucleoli contain roughly equivalent amounts of RNA, somewhat less than is present in the nuclear sap. The RNA/DNA ratio of the whole chromosomes is about 10. However, the ratio in the synthetically active regions, the loops, is much higher, since the loops contain all the chromosomal RNA but only a small fraction of the DNA.

Keywords

ADENINE CELL NUCLEUS CHROMOSOMES CYTOSINE ELECTROPHORESIS EXPERIMENTAL LAB STUDY GERM CELLS GUANINE RNA SALAMANDERS URACIL

MeSH Terms

Adenine Animals Base Composition Cell Nucleolus Cell Nucleus Chromatin Chromosomes Cytoplasm Cytosine DNA Electrophoresis Germ Cells Guanine Oocytes RNA Research Triturus Uracil Urodela

Chemicals

Chromatin Uracil Guanine RNA Cytosine DNA Adenine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

EDSTROEM J E

GALL J G

References (10)

10 references, click to expand

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Proc Natl Acad Sci U S A. 1962 Jul 15;48:1222-30 PMID: 14450185
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Proc Natl Acad Sci U S A. 1961 Oct 15;47:1611-9 PMID: 14491775
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Proc Natl Acad Sci U S A. 1962 Mar 15;48:471-7 PMID: 13912477
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Article Info

Journal

The Journal of cell biology

Abbr.

J Cell Biol

ISSN

0021-9525

DOI

10.1083/jcb.19.2.279

Published

1963-11-00

Pages

279-84

Language

English

Region

United States

NLM ID

0375356

PMCID

PMC2106876

Subset

External Links

PubMed source DOI: 10.1083/jcb.19.2.279 PMC Full Text View journal details

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THE BASE COMPOSITION OF RIBONUCLEIC ACID IN LAMPBRUSH CHROMOSOMES, NUCLEOLI, NUCLEAR SAP, AND CYTOPLASM OF TRITURUS OOCYTES.

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