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PMID: 1408792 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Repair of UV-induced (6-4)photoproducts measured in individual genes in the Drosophila embryonic Kc cell line.

Nucleic acids research ·Vol. 20 ·No. 18 ·1992-09-25 ·Pages 4789-93

de Cock JG, van Hoffen A, Wijnands J, Molenaar G, Lohman PH, Eeken JC

Abstract

The nucleotide excision repair (NER; dark-repair) of (6-4)photoproducts ((6-4)PPs) was assayed in cells from a permanent Drosophila melanogaster embryonic cell line, Kc, after exposure to 20 or 40 J/m2 ultraviolet (UV) light. Induction rates in the transcriptionally active genes Gart and Notch as well as in the inactive white locus is similar. They are formed with a frequency of about one-third of that of cyclobutane pyrimidine dimers (CPDs). In all three genes, (6-4)PPs are repaired with the same rate and to the same extent: 31% of the (6-4)PPs are removed in 4 hours post-irradiation and after 16 hours repair is nearly complete. In none of the three genes strand-specific repair was found. Exposure of cells that were irradiated with 40 J/m2 UV to photoreactivating light for 1 hour prior to dark-repair incubation, resulted in enhanced repair of (6-4)PPs.

MeSH Terms
Animals Cell Line DNA/radiation effects DNA Damage DNA Repair Deoxyribonuclease (Pyrimidine Dimer) Drosophila melanogaster Embryo, Nonmammalian Endodeoxyribonucleases Escherichia coli Proteins Genes, Insect/radiation effects Pyrimidine Dimers Restriction Mapping Ultraviolet Rays
Chemicals
Escherichia coli Proteins Pyrimidine Dimers DNA Endodeoxyribonucleases endodeoxyribonuclease uvrABC Deoxyribonuclease (Pyrimidine Dimer)
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
de Cock J G
MGC-Department of Radiation Genetics and Chemical Mutagenesis, State University of Leiden, The Netherlands.
van Hoffen A
Wijnands J
Molenaar G
Lohman P H
Eeken J C
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/20.18.4789
Published
1992-09-25
Pages
4789-93
Language
English
Region
England
NLM ID
0411011
PMCID
PMC334233
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/20.18.4789 PMC Full Text View journal details
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