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PMID: 14066138 Published · ppublish English Journal Article

THE MODE OF ACTION OF NEOSTIGMINE AND PHYSOSTIGMINE ON THE GUINEA-PIG TRACHEALIS MUSCLE.

British journal of pharmacology and chemotherapy ·Vol. 21 ·1963-08-00 ·Pages 137-49

CARLYLE RF

Abstract

Neostigmine (2.2 mug/ml.) or physostigmine (3.3 mug/ml.) contracted guinea-pig isolated tracheal chains. The anticholinesterase diisopropylphosphodiamidic fluoride (mipafox) itself caused no contractile response even in concentrations of 100 mug/ml., yet neostigmine or physostigmine still caused contractions after treatment with mipafox. The responses were abolished by hyoscine or atropine. Local anaesthetics, cooling, ionic changes and hemicholinium, all known to inhibit the release of acetylcholine from nerve endings, abolished or much reduced the responses. It seems that the contractile response to physostigmine or neostigmine does not depend on their anticholinesterase activity, but on their ability to release acetylcholine from postganglionic parasympathetic nerve endings.

Keywords
EXPERIMENTAL LAB STUDY GUINEA PIGS NEOSTIGMINE PHARMACOLOGY PHYSOSTIGMINE TRACHEA
MeSH Terms
Acetylcholine Animals Atropine Cholinesterase Inhibitors Guinea Pigs Muscle Contraction Muscle, Smooth Neostigmine Pharmacology Physostigmine Research Scopolamine Trachea
Chemicals
Cholinesterase Inhibitors Neostigmine Atropine Physostigmine Scopolamine Acetylcholine
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
CARLYLE R F
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25 references, click to expand
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Article Info
Journal
British journal of pharmacology and chemotherapy
Abbr.
Br J Pharmacol Chemother
ISSN
0366-0826
DOI
10.1111/j.1476-5381.1963.tb01509.x
Published
1963-08-00
Pages
137-49
Language
English
Region
England
NLM ID
0154627
PMCID
PMC1703869
Subset
OM
External Links
PubMed source DOI: 10.1111/j.1476-5381.1963.tb01509.x PMC Full Text
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