IDENTIFICATION OF CORYNEBACTERIUM DIPHTHERIAE WITH FLUORESCENT ANTIBACTERIAL REAGENTS.

Journal of bacteriology ·Vol. 86 ·1963-08-00 ·Pages 285-93

MOODY MD, JONES WL

Abstract

Moody, Max D. (Communicable Disease Center, Atlanta, Ga.) and Wallis L. Jones. Identification of Corynebacterium diphtheriae with fluorescent antibacterial reagents. J. Bacteriol. 86:285-293. 1963.-Conditions are described whereby fluorescent-antibody reagents could be prepared and used to identify Corynebacterium diphtheriae in pure and mixed cultures and in clinical materials. The use of O and OK antigens for immunization of rabbits to prepare the antibody was compared. The most satisfactory reagents were those made from serum of rabbits injected with live (OK) suspensions of C. diphtheriae. Such fluorescent reagents were used successfully in direct and indirect fluorescent-antibody tests to identify both toxinogenic and atoxinogenic C. diphtheriae but not to differentiate the two kinds of organisms.

Keywords

CORYNEBACTERIUM DIPHTHERIAE EXPERIMENTAL LAB STUDY FLUORESCENT ANTIBODY TECHNIC

MeSH Terms

Animals Anti-Bacterial Agents Coloring Agents Corynebacterium diphtheriae Fluorescent Antibody Technique Indicators and Reagents Rabbits Research

Chemicals

Anti-Bacterial Agents Coloring Agents Indicators and Reagents

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

MOODY M D

JONES W L

References (14)

14 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.86.2.285-293.1963

Published

1963-08-00

Pages

285-93

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC278421

Subset

External Links

PubMed source DOI: 10.1128/jb.86.2.285-293.1963 PMC Full Text View journal details

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IDENTIFICATION OF CORYNEBACTERIUM DIPHTHERIAE WITH FLUORESCENT ANTIBACTERIAL REAGENTS.

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