MALATE DEHYDROGENASE: MULTIPLE FORMS IN SEPARATED BLASTOMERES OF SEA URCHIN EMBRYOS.

Science (New York, N.Y.) ·Vol. 142 ·No. 3590 ·1963-10-18 ·Pages 389-90

MOORE RO, VILLEE CA

Abstract

Sea urchin embryos at the 64-cell stage were dissociated by treatment with trypsin and separated by centrifugation on a sucrose gradient. The large blastomeres have two and the small blastomeres have three bands of L-malate dehydrogenase activity, which are separated by disk microelectrophoresis on polyacrylamide gel, whereas unfertilized eggs have five.

Keywords

ECHINODERMATA EMBRYO EXPERIMENTAL LAB STUDY MALATE DEHYDROGENASE

MeSH Terms

Animals Blastomeres Echinodermata Embryo, Mammalian Embryo, Nonmammalian Malate Dehydrogenase Ovum Research Sea Urchins

Chemicals

Malate Dehydrogenase

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

MOORE R O

VILLEE C A

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.142.3590.389

Published

1963-10-18

Pages

389-90

Language

English

Region

United States

NLM ID

0404511

Subset

External Links

PubMed source DOI: 10.1126/science.142.3590.389 View journal details

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MALATE DEHYDROGENASE: MULTIPLE FORMS IN SEPARATED BLASTOMERES OF SEA URCHIN EMBRYOS.

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