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PMID: 1404388 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The repertoire of human germline VH sequences reveals about fifty groups of VH segments with different hypervariable loops.

Journal of molecular biology ·Vol. 227 ·No. 3 ·1992-10-05 ·Pages 776-98

Tomlinson IM, Walter G, Marks JD, Llewelyn MB, Winter G

Abstract

We have used the polymerase chain reaction and VH family-based primers to clone and sequence 74 human germline VH segments from a single individual and built a directory to include all known germline sequences. The directory contains 122 VH segments with different nucleotide sequences, 83 of which have open reading frames. The directory indicates that the structural diversity of the germline repertoire for antigen binding is fixed by about 50 groups of VH segments: each group encodes identical hypervariable loops. The directory should help in mapping the VH locus, in estimating somatic mutation and VH segment usage and in designing and constructing synthetic antibody libraries.

MeSH Terms

Amino Acid Sequence Base Sequence Cloning, Molecular Gene Library Gene Rearrangement, B-Lymphocyte, Heavy Chain/genetics Genes, Immunoglobulin Humans Immunoglobulin Heavy Chains/chemistry,genetics Immunoglobulin Variable Region/chemistry,genetics Molecular Sequence Data Oligonucleotide Probes/genetics Polymerase Chain Reaction

Chemicals

Immunoglobulin Heavy Chains Immunoglobulin Variable Region Oligonucleotide Probes

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Tomlinson I M

MRC Centre for Protein Engineering, Cambridge, U.K.

Walter G

Marks J D

Llewelyn M B

Winter G

Article Info

Journal

Journal of molecular biology

Abbr.

J Mol Biol

ISSN

0022-2836

DOI

10.1016/0022-2836(92)90223-7

Published

1992-10-05

Pages

776-98

Language

English

Region

England

NLM ID

2985088R

Subset

Databases

GENBANK

Z12303, Z12304, Z12305, Z12306, Z12307, Z12308, Z12309, Z12310, Z12311, Z12312, Z12313, Z12314, Z12315, Z12316, Z12317, Z12318, Z12319, Z12320, Z12321, Z12322, Z12323, Z12324, Z12325, Z12326, Z12327, Z12328, Z12329, Z12330, Z12331, Z12332

External Links

PubMed source DOI: 10.1016/0022-2836(92)90223-7 View journal details

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The repertoire of human germline VH sequences reveals about fifty groups of VH segments with different hypervariable loops.

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