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PMID: 1403813 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Sarcoplasmic reticulum buffering of myoplasmic calcium in bovine coronary artery smooth muscle.

The Journal of physiology ·Vol. 451 ·1992-00-00 ·Pages 25-48

Sturek M, Kunda K, Hu Q

Abstract

1. We tested the hypothesis that the sarcoplasmic reticulum (SR) buffers (attenuates) the increase in averaged myoplasmic free [Ca2+] (Ca(im)) resulting from Ca2+ influx. 2. Fura-2 measurements of Ca(im) were obtained in single smooth muscle cells freshly dispersed from bovine coronary artery. 3. Caffeine (5 x 10(-3) M) elicited a transient increase in Ca(im) and depleted the SR Ca2+ store. In the continued presence of caffeine or 10(-5) M-ryanodine SR buffering of Ca(im) was inhibited. Subsequent exposure to high extracellular [K+] (greater than 30 mM, equimolar Na+ removal) elicited a 2-fold more rapid and 2-fold greater peak increase in Ca(im) than high K+ elicited when SR buffering of Ca(im) was normal. The augmented increase in Ca(im) was inhibited 35% by 10(-5) M-diltiazem, 65% by 2 x 10(-4) M-LaCl3, and 87% in Ca(2+)-free external solution. 4. When Ca(im) buffering capacity was increased by partially depleting the SR with a transient (1 min) exposure to caffeine, subsequent exposure to 80 nM-K+ solution increased Ca(im) almost 2-fold more slowly than 80 mM-K+ before depletion of Ca2+ from the SR. However, the influxing Ca2+ was sequestered by the SR and refilled it, as evident by the subsequent caffeine-induced Ca(im) transient being identical to the first. Increasing extracellular [K+] (thus, increasing depolarization and Na+ removal) caused proportional increases in Ca(im) and the subsequent caffeine-induced Ca(im) transients were proportionally larger, indicating a graded filling of the SR by Ca2+ influx. 5. Diltiazem (10(-5) M) inhibited the refilling of the SR achieved by 80 mM-K+, by 26%. Refilling was inhibited 76% by 80 mM-K+, Ca(2+)-free solution, indicating the fraction of refilling dependent on influx of Ca2+ through voltage-gated Ca2+ channels, leak channels, and other influx pathways. Mild depolarization with 35 mM-K+ (no Na+ removal) often caused no increase in Ca(im), but influx through voltage-gated Ca2+ channels occurred because the SR Ca2+ store was refilled. Also, 10(-5) M-diltiazem or 10(-6) M-TA3090 inhibited the refilling to levels attributable only to leak influx of Ca2+. 6. All data support our hypothesis that the SR significantly attenuates the amount of Ca2+ influx that accumulates to increase Ca(im).(ABSTRACT TRUNCATED AT 400 WORDS)

MeSH Terms
Animals Buffers Caffeine/pharmacology Calcium/metabolism Cattle Coronary Vessels/metabolism Diltiazem/pharmacology Extracellular Space/metabolism Fura-2 In Vitro Techniques Ion Transport/drug effects Muscle, Smooth, Vascular/drug effects,metabolism Myofibrils/metabolism Potassium/metabolism Sarcoplasmic Reticulum/drug effects,metabolism
Chemicals
Buffers Caffeine Diltiazem Potassium Calcium Fura-2
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sturek M
Department of Physiology, School of Medicine, University of Missouri, Columbia 65211.
Kunda K
Hu Q
References (52)
52 references, click to expand
  1. A model for receptor-regulated calcium entry.
    Cell Calcium. 1986 Feb;7(1):1-12 PMID: 2420465
  2. A comparison of calcium-activated potassium channel currents in cell-attached and excised patches.
    J Gen Physiol. 1987 Jun;89(6):985-97 PMID: 2440980
  3. Drug-induced Ca2+ release from isolated sarcoplasmic reticulum. I. Use of pyrophosphate to study caffeine-induced Ca2+ release.
    J Biol Chem. 1987 May 5;262(13):6135-41 PMID: 2437114
  4. Purified ryanodine receptor from skeletal muscle sarcoplasmic reticulum is the Ca2+-permeable pore of the calcium release channel.
    J Biol Chem. 1987 Dec 5;262(34):16636-43 PMID: 2445748
  5. Nitrendipine inhibition of calcium current in rat vascular muscle cells.
    J Cardiovasc Pharmacol. 1988;12 Suppl 5:S100-3 PMID: 2469865
  6. Desensitization of swine arterial smooth muscle to transplasmalemmal Ca2+ influx.
    J Physiol. 1989 Sep;416:273-90 PMID: 2558174
  7. Macroscopic K+ currents in single smooth muscle cells of the rabbit portal vein.
    J Physiol. 1989 Jun;413:49-73 PMID: 2600861
  8. Effects of activation on distribution of Ca2+ in single arterial smooth muscle cells. Determination with fura-2 digital imaging microscopy.
    Circ Res. 1989 May;64(5):1019-29 PMID: 2706759
  9. Mobilization of free Ca2+ measured during contraction-relaxation cycles in smooth muscle cells of the porcine coronary artery using quin2.
    Pflugers Arch. 1986 Feb;406(2):173-80 PMID: 3083398
  10. Measurement of neuronal Ca2+ transients using simultaneous microfluorimetry and electrophysiology.
    Pflugers Arch. 1988 Jul;412(1-2):216-23 PMID: 3174384
  11. Quin2 microfluorometry and effects of verapamil and diltiazem on calcium release from rat aorta smooth muscle cells in primary culture.
    Circ Res. 1988 Jul;63(1):16-26 PMID: 3383374
  12. Alterations in cytoplasmic calcium sensitivity during porcine coronary artery contractions as detected by aequorin.
    J Physiol. 1987 Apr;385:437-48 PMID: 3477639
  13. Spontaneous transient outward currents in single visceral and vascular smooth muscle cells of the rabbit.
    J Physiol. 1986 Dec;381:385-406 PMID: 2442353
  14. Calcium ions, active zones and synaptic transmitter release.
    Trends Neurosci. 1988 Oct;11(10):458-64 PMID: 2469165
  15. Agonist-induced vascular tone.
    Hypertension. 1989 Jun;13(6 Pt 2):835-44 PMID: 2544524
  16. Sodium-calcium exchange current. Dependence on internal Ca and Na and competitive binding of external Na and Ca.
    J Gen Physiol. 1989 Jun;93(6):1129-45 PMID: 2549177
  17. Calcium-force coupling mechanisms during vasodilator-induced relaxation of ferret aorta.
    J Physiol. 1989 May;412:123-33 PMID: 2600829
  18. Cellular mechanisms regulating [Ca2+]i smooth muscle.
    Annu Rev Physiol. 1989;51:315-29 PMID: 2653185
  19. Current fluctuations and oscillations in smooth muscle cells from hog carotid artery. Role of the sarcoplasmic reticulum.
    Circ Res. 1989 Sep;65(3):708-22 PMID: 2766490
  20. Vascular smooth muscle sarcoplasmic reticulum. Function and mechanisms of Ca2+ release.
    Ann N Y Acad Sci. 1988;522:60-73 PMID: 2837123
  21. Simultaneous measurement of calcium transients and motion in cultured heart cells.
    Am J Physiol. 1987 Dec;253(6 Pt 2):H1400-8 PMID: 3122589
  22. Ryanodine: its possible mechanism of action in the caffeine-sensitive calcium store of smooth muscle.
    Pflugers Arch. 1988 Sep;412(4):376-81 PMID: 3174395
  23. Fura-2 calcium transients in frog skeletal muscle fibres.
    J Physiol. 1988 Sep;403:151-92 PMID: 3267019
  24. Myoplasmic [Ca2+] determines myosin phosphorylation in agonist-stimulated swine arterial smooth muscle.
    Circ Res. 1988 Sep;63(3):593-603 PMID: 3409490
  25. A Ca2+-insensitive form of fura-2 associated with polymorphonuclear leukocytes. Assessment and accurate Ca2+ measurement.
    J Biol Chem. 1987 May 5;262(13):6308-12 PMID: 3571258
  26. Calcium rises abruptly and briefly throughout the cell at the onset of anaphase.
    Science. 1986 Aug 22;233(4766):886-9 PMID: 3755550
  27. A new generation of Ca2+ indicators with greatly improved fluorescence properties.
    J Biol Chem. 1985 Mar 25;260(6):3440-50 PMID: 3838314
  28. Sarcoplasmic reticulum and excitation-contraction coupling in mammalian smooth muscles.
    J Cell Biol. 1972 Mar;52(3):690-718 PMID: 5061887
  29. Calcium messenger system: an integrated view.
    Physiol Rev. 1984 Jul;64(3):938-84 PMID: 6330778
  30. Characterization of single isolated vascular smooth muscle cells from bovine coronary artery.
    Blood Vessels. 1984;21(6):267-78 PMID: 6498341
  31. Effect of acute coronary artery occlusion on local myocardial extracellular K+ activity in swine.
    Circulation. 1980 Apr;61(4):768-78 PMID: 7357719
  32. Depolarization-stimulated 42K+ efflux in rat aorta is calcium- and cellular volume-dependent.
    Circ Res. 1987 Jul;61(1):1-11 PMID: 3608107
  33. Calcium gradients in single smooth muscle cells revealed by the digital imaging microscope using Fura-2.
    Nature. 1985 Dec 12-18;318(6046):558-61 PMID: 3934562
  34. Mechanism of 5-HT contraction in isolated bovine ventricular coronary arteries. Evidence for transient receptor-operated calcium influx channels.
    Circ Res. 1984 Feb;54(2):135-43 PMID: 6319042
  35. Exchange characteristics of the noradrenaline-sensitive calcium store in vascular smooth muscle cells or rabbit ear artery.
    J Physiol. 1981 Aug;317:263-79 PMID: 7310734
  36. Calculator programs for computing the composition of the solutions containing multiple metals and ligands used for experiments in skinned muscle cells.
    J Physiol (Paris). 1979;75(5):463-505 PMID: 533865
  37. Spontaneous sarcoplasmic reticulum calcium release and extrusion from bovine, not porcine, coronary artery smooth muscle.
    J Physiol. 1992;451:49-78 PMID: 1403820
  38. Contribution of two types of calcium channels to membrane conductance of single myocytes from guinea-pig coronary artery.
    J Physiol. 1990 Jul;426:19-42 PMID: 1700105
  39. Endothelin mediates Ca influx and release in porcine coronary smooth muscle cells.
    Am J Physiol. 1991 Apr;260(4 Pt 1):C771-7 PMID: 1850196
  40. Sodium current-induced release of calcium from cardiac sarcoplasmic reticulum.
    Science. 1990 Apr 20;248(4953):372-6 PMID: 2158146
  41. Endothelin augments unitary calcium channel currents on the smooth muscle cell membrane of guinea-pig portal vein.
    J Physiol. 1990 Apr;423:171-91 PMID: 2167361
  42. Agonist-dependent Ca2+ and Mn2+ entry dependent on state of filling of Ca2+ stores in aortic smooth muscle cells of the rat.
    J Physiol. 1990 Aug;427:171-86 PMID: 2213595
  43. Exercise training alters Ca release from coronary smooth muscle sarcoplasmic reticulum.
    Am J Physiol. 1990 Aug;259(2 Pt 2):H643-7 PMID: 2386234
  44. Acetylcholine- and 5-hydroxytryptamine-stimulated contraction and calcium uptake in bovine coronary arteries: evidence for two populations of receptor-operated calcium channels.
    J Pharmacol Exp Ther. 1985 Sep;234(3):641-7 PMID: 2411912
  45. Calcium requirement for activation of intact aortic smooth muscle.
    J Physiol. 1977 Nov;272(2):317-29 PMID: 201748
  46. Primary action of endothelin on Ca release in bovine coronary artery smooth muscle cells.
    Am J Physiol. 1991 Apr;260(4 Pt 1):C763-70 PMID: 1708203
  47. Thapsigargin, a tumor promoter, discharges intracellular Ca2+ stores by specific inhibition of the endoplasmic reticulum Ca2(+)-ATPase.
    Proc Natl Acad Sci U S A. 1990 Apr;87(7):2466-70 PMID: 2138778
  48. Comparison of endothelin- and caffeine-induced calcium mobilization in coronary artery smooth muscle cells.
    Prog Clin Biol Res. 1990;327:517-24 PMID: 2181473
  49. Sodium-calcium exchange in excitable cells: fuzzy space.
    Science. 1990 Apr 20;248(4953):283 PMID: 2326638
  50. Calcium channels in muscle cells isolated from rat mesenteric arteries: modulation by dihydropyridine drugs.
    Circ Res. 1986 Aug;59(2):229-35 PMID: 2427250
  51. Cellular calcium regulates outward currents in rabbit intestinal smooth muscle cell.
    Am J Physiol. 1987 Apr;252(4 Pt 1):C401-10 PMID: 2436483
  52. Calcium antagonism and calcium entry blockade.
    Pharmacol Rev. 1986 Dec;38(4):321-416 PMID: 2432624
Article Info
Journal
The Journal of physiology
Abbr.
J Physiol
ISSN
0022-3751
DOI
10.1113/jphysiol.1992.sp019152
Published
1992-00-00
Pages
25-48
Language
English
Region
England
NLM ID
0266262
PMCID
PMC1176149
Subset
IM
Grants
NHLBI NIH HHS · HL41033 · United States
NCRR NIH HHS · RR 07053 · United States
External Links
PubMed source DOI: 10.1113/jphysiol.1992.sp019152 PMC Full Text View journal details
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