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PMID: 1400258 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Resolution of chromosomes III and VI of Aspergillus nidulans by pulsed-field gel electrophoresis shows that the penicillin biosynthetic pathway genes pcbAB, pcbC, and penDE are clustered on chromosome VI (3.0 megabases).

Journal of bacteriology ·Vol. 174 ·No. 21 ·1992-11-00 ·Pages 7063-7

Montenegro E, Fierro F, Fernandez FJ, Gutiérrez S, Martín JF

Abstract

An improved electrophoretic molecular karyotype of Aspergillus nidulans ATCC 28901 has been obtained by contour-clamped electric field gel electrophoresis, which separates seven chromosomal bands and allows resolution of chromosomes III and VI. The three genes of the penicillin biosynthetic pathway, pcbAB, pcbC, and penDE, encoding alpha-aminoadipyl-cysteinyl-valine synthetase, isopenicillin N synthase, and isopenicillin N acyltransferase, respectively, are clustered together on a chromosome of 3.0 Mg, corresponding to linkage group VI, whereas the argB gene was located on a chromosome of 3.4 Mb, corresponding to linkage group III. Three other strains of A. nidulans contained a modified chromosome III of about 3.1 Mb that overlaps with chromosome VI, forming a doublet. Resolution of chromosomes III and VI in strain ATCC 28901 allowed unequivocal mapping of the penicillin gene cluster on chromosome VI of A. nidulans.

MeSH Terms
Acyltransferases/genetics Aspergillus nidulans/genetics Chromosome Mapping Chromosomes, Fungal Electrophoresis, Gel, Pulsed-Field Genes, Fungal/genetics Karyotyping/methods Oxidoreductases/genetics Penicillin-Binding Proteins Penicillins/biosynthesis Peptide Synthases/genetics
Chemicals
Penicillin-Binding Proteins Penicillins Oxidoreductases isopenicillin N synthetase Acyltransferases acyl-CoA-6-aminopenicillanic acid acyltransferase Peptide Synthases alpha-aminoadipyl-cysteinyl-valine synthetase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Montenegro E
Department of Ecology, Genetics and Microbiology, Faculty of Biology, University of León, Spain.
Fierro F
Fernandez F J
Gutiérrez S
Martín J F
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.174.21.7063-7067.1992
Published
1992-11-00
Pages
7063-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC207390
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.174.21.7063-7067.1992 PMC Full Text View journal details
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