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PMID: 1398938 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Role of Yersinia enterocolitica Yst toxin in experimental infection of young rabbits.

Infection and immunity ·Vol. 60 ·No. 10 ·1992-10-00 ·Pages 4269-77

Delor I, Cornelis GR

Abstract

We constructed a Yst-negative mutant of Yersinia enterocolitica W1024 by reverse genetics, and we compared the virulence of the yst+ and yst isogenic strains in an experimental oral infection of the young rabbit. The rabbits infected with the yst+ strain suffered from the diarrhea and lost weight, and most of them died. By contrast, the occurrence of diarrhea, weight loss, and death in the group of rabbits infected with the yst mutant was as low as that in the group of uninfected rabbits. Bacteria from both strains were excreted in the feces and induced a serum antibody response against Yop proteins. The yst mutant disappeared more rapidly from the feces. We conclude that the enterotoxin Yst is a major factor involved in the Y. enterocolitica-associated diarrhea in the young rabbit. Given the similarity with the symptoms observed for children, this result suggests that Yst could also be an important factor in diarrhea in young children infected with Y. enterocolitica.

MeSH Terms
Animals Bacterial Toxins/toxicity Female Male Mutation Rabbits Yersinia Infections/etiology Yersinia enterocolitica/pathogenicity
Chemicals
Bacterial Toxins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Delor I
Microbial Pathogenesis Unit, International Institute of Cellular and Molecular Pathology, Brussels, Belgium.
Cornelis G R
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.60.10.4269-4277.1992
Published
1992-10-00
Pages
4269-77
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC257462
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.60.10.4269-4277.1992 PMC Full Text View journal details
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