Physicochemical studies on the reaction between formaldehyde and DNA.

Biophysical journal ·Vol. 3 ·1963-01-00 ·Pages 49-63

FREIFELDER D, DAVISON PF

Abstract

The reaction between formaldehyde and phage T7 DNA has been studied by optical absorbance and sedimentation measurements. Through the course of denaturation, OD(200) and s(20, w) rise; after the attainment of full hyperchromicity the s(20, w) falls sharply, suggesting a decrease in molecular weight. Conditions in which formaldehyde causes cross-linking are defined. Some experimental applications of the denaturation technique are given. Evidence which suggests that preformed single-strand interruptions may exist in phage DNA is briefly discussed.

Keywords

COLIPHAGES DNA VIRAL FORMALDEHYDE

MeSH Terms

Coliphages DNA DNA, Viral Formaldehyde Molecular Weight

Chemicals

DNA, Viral Formaldehyde DNA

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

FREIFELDER D

DAVISON P F

References (15)

15 references, click to expand

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Article Info

Journal

Biophysical journal

Abbr.

Biophys J

ISSN

0006-3495

DOI

10.1016/s0006-3495(63)86803-4

Published

1963-01-00

Pages

49-63

Language

English

Region

United States

NLM ID

0370626

PMCID

PMC1366423

Subset

External Links

PubMed source DOI: 10.1016/s0006-3495(63)86803-4 PMC Full Text View journal details

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Physicochemical studies on the reaction between formaldehyde and DNA.

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