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PMID: 1393282 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Ca(2+)-stores mobilization by diadenosine tetraphosphate, Ap4A, through a putative P2Y purinoceptor in adrenal chromaffin cells.

British journal of pharmacology ·Vol. 106 ·No. 4 ·1992-08-00 ·Pages 833-7

Castro E, Pintor J, Miras-Portugal MT

Abstract

1. Diadenosine tetraphosphate (Ap4A) evoked a concentration-dependent increase in cytosolic [Ca2+] in resting chromaffin cells. The EC50 value for this action was 28.2 +/- 6.6 microM. This effect was also produced by diadenosine pentaphosphate (Ap5A) with an EC50 of 50 +/- 7 microM. 2. In contrast with this effect, pretreatment with Ap4A or Ap5A induced a 30% reduction in Ca2+ entry following 10 microM dimethylphenylpiperazinium. 3. The elevation in cytosolic [Ca2+] induced by Ap4A was persistent in approximately 100 nM external [Ca2+] and was sensitive to depletion of internal Ca2+ stores by a bradykinin prepulse or whole cell depletion in Ca2+. 4. The effect of Ap4A was mimicked and desensitized by the agonist adenosine 5'-O-(2-thiodiphosphate), and blocked by the P2Y-receptor antagonist, cibachrome blue. The P2X-receptor agonist alpha,beta-methylene adenosine 5'-triphosphate was inactive both by itself or in combination with Ap4A. This is compatible with a P2Y-purinoceptor-mediated action.

MeSH Terms
Adrenal Glands/physiology Bradykinin/pharmacology Calcium/metabolism Chromaffin Granules/physiology Dinucleoside Phosphates/pharmacology Dose-Response Relationship, Drug In Vitro Techniques Receptors, Purinergic/drug effects Second Messenger Systems/physiology
Chemicals
Dinucleoside Phosphates Receptors, Purinergic diadenosine tetraphosphate Bradykinin Calcium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Castro E
Departamento de Bioquímica, Facultad de Veterinaria, Universidad Complutense de Madrid, España.
Pintor J
Miras-Portugal M T
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Article Info
Journal
British journal of pharmacology
Abbr.
Br J Pharmacol
ISSN
0007-1188
DOI
10.1111/j.1476-5381.1992.tb14421.x
Published
1992-08-00
Pages
833-7
Language
English
Region
England
NLM ID
7502536
PMCID
PMC1907636
Subset
IM
External Links
PubMed source DOI: 10.1111/j.1476-5381.1992.tb14421.x PMC Full Text View journal details
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