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PMID: 1387083 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The carboxy-terminal exon of the adenovirus E1A protein is required for E4F-dependent transcription activation.

The EMBO journal ·Vol. 11 ·No. 9 ·1992-09-00 ·Pages 3347-54

Bondesson M, Svensson C, Linder S, Akusjärvi G

Abstract

The adenovirus-2 E1A 289R transcription activator protein contains a 49 amino acid sequence (designated CR3) that has been suggested to represent the minimal domain required for E1A-induced activation of viral early transcription. We show here that the non-conserved carboxy-terminal E1A exon contains two interchangeable elements that are required for efficient CR3-dependent transactivation of the adenovirus E4 promoter in HeLa cells. These two elements do not encode independent transactivation functions and have been designated auxiliary regions (ARs) 1 and 2. The effects of AR1 and AR2 are not additive, suggesting that they contribute a mechanistically analogous function in transcription. Previous studies have suggested that two cellular transcription factors, ATF-2 and E4F, can function together with E1A to induce transcription of the E4 promoter. The importance of respective factors for E4 transcription has not been resolved. We find that E1A activation of E4F, but not ATF-2 (or other ATF factors), is AR1- and AR2-dependent. This result suggests that E1A induction of the E4 promoter in HeLa cells is primarily mediated by E4F.

Related Genes
E1A
MeSH Terms
Activating Transcription Factors Adenoviridae/genetics Adenovirus Early Proteins Amino Acid Sequence Blood Proteins/metabolism DNA Mutational Analysis Exons Gene Expression Regulation, Viral HeLa Cells Humans Molecular Sequence Data Oncogene Proteins, Viral/genetics,metabolism Promoter Regions, Genetic Protein Conformation Recombinant Fusion Proteins Repetitive Sequences, Nucleic Acid Sequence Homology, Nucleic Acid Structure-Activity Relationship Trans-Activators/genetics,metabolism Transcription Factors/metabolism Transcription, Genetic
Chemicals
Activating Transcription Factors Adenovirus Early Proteins Blood Proteins Oncogene Proteins, Viral Recombinant Fusion Proteins Trans-Activators Transcription Factors
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bondesson M
Department of Microbial Genetics, Karolinska Institute, Stockholm, Sweden.
Svensson C
Linder S
Akusjärvi G
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45 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1992-09-00
Pages
3347-54
Language
English
Region
England
NLM ID
8208664
PMCID
PMC556869
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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