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PMID: 13864610 Published · ppublish English Journal Article

Antigens of Bordetella pertussis. II. Purification of heat-labile toxin.

Journal of bacteriology ·Vol. 84 ·1962-08-00 ·Pages 269-74

BANERJEA A, MUNOZ J

Abstract

Banerjea, A. (Rocky Mountain Laboratory, Hamilton, Mont.) and J. Munoz. Antigens of Bordetella pertussis. II, Purification of heat-labile toxin. J. Bacteriol. 84:269-274. 1962.-A mild method of separating heat-labile toxin of Bordetella pertussis from other cellular components is described; it consists of absorbing toxin on a diethylaminoethyl cellulose column and eluting it with a gradient concentration of NaCl. Toxin preparations thus obtained consisted mainly of protein; their toxicity was destroyed by trypsin but not by ribonuclease or deoxyribonuclease.

Keywords
ANTIGENS BORDETELLA PERTUSSIS/immunology TOXINS AND ANTITOXINS
MeSH Terms
Antigens Antitoxins Bordetella pertussis/immunology Hot Temperature Toxins, Biological
Chemicals
Antigens Antitoxins Toxins, Biological
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
BANERJEA A
MUNOZ J
References (10)
10 references, click to expand
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    J Immunol. 1959 Nov;83:496-501 PMID: 14425265
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    Pediatrics. 1955 Nov;16(5):637-51 PMID: 13273100
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    Proc Soc Exp Biol Med. 1950 May;74(1):75-8 PMID: 15430395
  10. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.84.2.269-274.1962
Published
1962-08-00
Pages
269-74
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC277853
Subset
OM
External Links
PubMed source DOI: 10.1128/jb.84.2.269-274.1962 PMC Full Text View journal details
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