Cloning, sequencing, and expression of the Escherichia coli peptide methionine sulfoxide reductase gene.

The Journal of biological chemistry ·Vol. 267 ·No. 22 ·1992-08-05 ·Pages 15549-51

Rahman MA, Nelson H, Weissbach H, Brot N

Abstract

The gene encoding peptide methionine sulfoxide reductase was cloned from an Escherichia coli genomic library using an oligonucleotide probe based on the amino-terminal sequence of the protein. The nucleotide sequence revealed that the gene codes for a polypeptide of 212 amino acid residues with a calculated molecular weight of 23,314. The protein has been overexpressed in E. coli and is present as a soluble active species.

MeSH Terms

Amino Acid Sequence Bacteriophage lambda/genetics Base Sequence Cloning, Molecular DNA Probes Escherichia coli/enzymology,genetics Genes, Bacterial Genomic Library Kinetics Methionine Sulfoxide Reductases Molecular Sequence Data Oligonucleotide Probes Oxidoreductases/genetics,metabolism Recombinant Proteins/metabolism

Chemicals

DNA Probes Oligonucleotide Probes Recombinant Proteins Oxidoreductases Methionine Sulfoxide Reductases methionine sulfoxide reductase

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Rahman M A

Roche Institute of Molecular Biology, Roche Research Center, Nutley, New Jersey 07110.

Nelson H

Weissbach H

Brot N

Article Info

Journal

The Journal of biological chemistry

Abbr.

J Biol Chem

ISSN

0021-9258

Published

1992-08-05

Pages

15549-51

Language

English

Region

United States

NLM ID

2985121R

Subset

Databases

GENBANK

M63383, M86615, M86616, M86617, M86618, M86619, M86620, M87862, M89992, X62322

External Links

PubMed source View journal details

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Cloning, sequencing, and expression of the Escherichia coli peptide methionine sulfoxide reductase gene.

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