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PMID: 1385446 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A point mutation of integrin beta 1 subunit blocks binding of alpha 5 beta 1 to fibronectin and invasin but not recruitment to adhesion plaques.

The Journal of cell biology ·Vol. 119 ·No. 4 ·1992-11-00 ·Pages 913-21

Takada Y, Ylänne J, Mandelman D, Puzon W, Ginsberg MH

Abstract

A point mutation in a highly conserved region of the beta 1 subunit, Asp130 to Ala (D130A) substitution, abrogates the Arg-Gly-Asp (RGD)-dependent binding of alpha 5 beta 1 to fibronectin (FN) without disrupting gross structure or heterodimer assembly. The D130A mutation also interferes with binding to invasin, a ligand that lacks RGD sequence. In spite of the lack of detectable FN binding by alpha 5 beta 1(D130A), it was recruited to adhesion plaques formed on FN by endogenous hamster receptors. Thus, intact ligand binding function is not required for recruitment of alpha 5 beta 1 to adhesion plaques. Overexpression of beta 1(D130A) partially interfered with endogenous alpha 5 beta 1 function, thus defining a dominant negative beta 1 integrin mutation.

MeSH Terms
Adhesins, Bacterial Amino Acid Sequence Animals Bacterial Proteins/metabolism Base Sequence CHO Cells Cell Adhesion Cricetinae Fibronectins/metabolism Integrin beta1 Integrins/genetics,metabolism Microscopy, Fluorescence Molecular Sequence Data Oligopeptides/metabolism Point Mutation Receptors, Fibronectin/metabolism
Chemicals
Adhesins, Bacterial Bacterial Proteins Fibronectins Integrin beta1 Integrins Oligopeptides Receptors, Fibronectin invasin, Yersinia arginyl-glycyl-aspartic acid
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Takada Y
Committee on Vascular Biology, Scripps Research Institute, La Jolla, CA 92037.
Ylänne J
Mandelman D
Puzon W
Ginsberg M H
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.119.4.913
Published
1992-11-00
Pages
913-21
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2289695
Subset
IM
Grants
NIAMS NIH HHS · AR27214 · United States
NIGMS NIH HHS · GM47157 · United States
NHLBI NIH HHS · HL28235 · United States
External Links
PubMed source DOI: 10.1083/jcb.119.4.913 PMC Full Text View journal details
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